Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,101,981	G→A	N49N (AAC→AAT)	csgF ←	curli assembly component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,101,981	0	G	A	100.0%	23.5 / NA	8	N49N (AAC→AAT)	csgF	curli assembly component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/4);  total (4/4)

CTGAGTAAAGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGA  >  NC_000913/1101909‑1102057                                                                         |                                                                             cTGAGTAAAGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAATTTTGGGc                                                                      <  2:221803/81‑1 (MQ=255)    aGTAAAGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAATTTTGGGcc                                                                     <  1:221804/79‑1 (MQ=255)                 aaCGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAATTTTGGGCCTGAGCGCTATTtaa                                                       >  1:221828/1‑80 (MQ=255)                 aaCGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAATTTTGGGCCTGAGCGCTATTtaa                                                       >  2:221821/1‑80 (MQ=255)                  aCGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAATTTTGGGCCTGAGCGCTATTtaata                                                     <  1:221805/81‑1 (MQ=255)                  aCGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAATTTTGGGCCTGAGCGCTATTtaa                                                       <  1:221806/79‑1 (MQ=255)                                                 gTTATAGCTCGGATCTTTATAAGAATTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTAccacca                        >  1:221826/1‑79 (MQ=255)                                                          cGGATCTTTATAAGAATTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGAt              >  1:221824/1‑80 (MQ=255)                                                                      agaaTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGa  >  1:221834/1‑80 (MQ=255)                                                                         |                                                                             CTGAGTAAAGTTATCTAACGCTGAGGGTGTTTCAATACCAAAGTCATCGTTATAGCTCGGATCTTTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGA  >  NC_000913/1101909‑1102057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑