Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,104,203	A→G	T85A (ACA→GCA)	csgB →	curlin, minor subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,104,203	0	A	G	100.0%	46.5 / NA	14	T85A (ACA→GCA)	csgB	curlin, minor subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/2);  total (12/2)

GTTACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGC  >  NC_000913/1104127‑1104273                                                                             |                                                                       gTTACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACcaggcagg                                                                    <  2:222036/81‑1 (MQ=255)   tACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAg                                                                     >  1:222050/1‑78 (MQ=255)   tACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAg                                                                     >  2:222057/1‑78 (MQ=255)           ggAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAAc                                                          >  1:222048/1‑81 (MQ=255)               gCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTg                                                      <  1:222037/81‑1 (MQ=255)                    aaaCTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCatat                                                 <  2:222038/81‑1 (MQ=255)                     aaCTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCatata                                                >  1:222054/1‑81 (MQ=255)                       cTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATAtt                                              >  1:222056/1‑81 (MQ=255)                               ggTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGc                                      >  1:222060/1‑81 (MQ=255)                                   gttgCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCggg                                   >  1:222064/1‑80 (MQ=255)                                        gcAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGc                             >  1:222063/1‑81 (MQ=255)                                            gaagGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAAc                         >  2:222053/1‑81 (MQ=255)                                              agGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGa                       >  1:222055/1‑81 (MQ=255)                                                     agCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAg                 >  2:222058/1‑80 (MQ=255)                                                                   gATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGc  >  2:222065/1‑81 (MQ=255)                                                                             |                                                                       GTTACGGCAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGC  >  NC_000913/1104127‑1104273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑