Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,125,850	G→A	V97I (GTT→ATT)	rimJ →	ribosomal‑protein‑S5‑alanine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,125,850	0	G	A	100.0%	72.8 / NA	21	V97I (GTT→ATT)	rimJ	ribosomal‑protein‑S5‑alanine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/15);  total (6/15)

AACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCA  >  NC_000913/1125773‑1125922                                                                              |                                                                         aaCAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAAtatt                                                                        <  2:225426/80‑1 (MQ=255)          cAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATATTGTTCGTGGc                                                               <  2:225427/80‑1 (MQ=255)           aGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATATTGTTCGTGGctc                                                             <  2:225428/81‑1 (MQ=255)                tCTACTTTGGCTTATTCGACCCGGACGAAGAAGAGATTATTGGCGTTGCCAATTTTTCCAATATTGTTCGTGGCTCttt                                                          <  2:225429/79‑1 (MQ=255)                   aCTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATg                                                     >  1:225458/1‑81 (MQ=255)                    cTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATg                                                     <  1:225430/80‑1 (MQ=255)                       tGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATACTGTTCGTGGCTCTTTTCATGcc                                                   <  2:225431/79‑1 (MQ=255)                        ggCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATGCCTGc                                                >  1:225465/1‑81 (MQ=255)                         gCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATGCCTGCt                                               <  1:225432/81‑1 (MQ=255)                                    ccGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTAt                                    <  2:225433/81‑1 (MQ=255)                                     cGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTAtt                                   <  2:225434/81‑1 (MQ=255)                                           aaaaaGAGATTATTGGCGTTGCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTg                             >  1:225456/1‑81 (MQ=255)                                               agagATTATTGGCGTTGCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTgggta                         <  2:225435/81‑3 (MQ=255)                                                   attattGGCGTTGCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCaaaaa                     <  2:225436/81‑1 (MQ=255)                                                    ttattGGCGTTGCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAAt                    <  1:225439/81‑1 (MQ=255)                                                    ttattGGCGTTGCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAAt                    <  1:225438/81‑1 (MQ=255)                                                    ttattGGCGTTGCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAAt                    <  2:225437/81‑1 (MQ=255)                                                         ggCGTTGCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAg               >  2:225463/1‑81 (MQ=255)                                                           cGTTGCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAggg             >  2:225462/1‑81 (MQ=255)                                                               gCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCaa          <  1:225440/80‑1 (MQ=255)                                                               gCCAATTTTTCCAATATTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCa           >  1:225460/1‑79 (MQ=255)                                                                       ttCCAATATTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCa  <  1:225441/80‑1 (MQ=255)                                                                              |                                                                         AACAAGGTTCAGCTTTCTACTTTGGCTTATTCGACCCGGACGAAAAAGAGATTATTGGCGTTGCCAATTTTTCCAATGTTGTTCGTGGCTCTTTTCATGCCTGCTATCTCGGTTATTCGATTGGGCAAAAATGGCAGGGCAAAGGACTCA  >  NC_000913/1125773‑1125922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑