Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,147,182	C→T	A130V (GCC→GTC)	yceD →	DUF177 domain‑containing protein YceD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,147,182	0	C	T	100.0%	122.5 / NA	34	A130V (GCC→GTC)	yceD	DUF177 domain‑containing protein YceD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/17);  total (17/17)

AAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGC  >  NC_000913/1147104‑1147256                                                                               |                                                                           aaGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCt                                                                          <  2:229248/81‑1 (MQ=255)     gTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGcc                                                                      >  1:229299/1‑81 (MQ=255)         gAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCgg                                                                    >  2:229285/1‑79 (MQ=255)         gAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGCTGAAGATGAAATCATCCTCGTCTTGCCgg                                                                    >  2:229307/1‑79 (MQ=255)           aCCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGt                                                                <  1:229249/81‑1 (MQ=255)             cGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTc                                                              >  2:229302/1‑81 (MQ=255)              gATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTcc                                                             <  1:229250/81‑1 (MQ=255)                ttGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCgg                                                           >  1:229301/1‑81 (MQ=255)                 tGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGt                                                          >  2:229296/1‑81 (MQ=255)                  gAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGt                                                          <  1:229253/80‑1 (MQ=255)                  gAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGt                                                          <  2:229252/80‑1 (MQ=255)                  gAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGt                                                          <  2:229251/80‑1 (MQ=255)                        aaCGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGAt                                                   >  2:229287/1‑81 (MQ=255)                         aCGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGAtt                                                  <  2:229254/81‑1 (MQ=255)                          cGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTc                                                 >  1:229298/1‑81 (MQ=255)                           gAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCt                                                <  2:229256/81‑1 (MQ=255)                           gAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCt                                                <  1:229255/81‑1 (MQ=255)                            aaTTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCt                                                >  1:229292/1‑80 (MQ=255)                                 ggTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAacac                                          >  1:229300/1‑81 (MQ=255)                                 ggTGAAATCGATCTGCTTGCAATGGTTGAAAATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAaca                                           <  2:229257/80‑1 (MQ=255)                                        tCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAg                                   <  1:229258/81‑1 (MQ=255)                                          gATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAgt                                  <  1:229259/80‑1 (MQ=255)                                                   gCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCg                        >  1:229312/1‑81 (MQ=255)                                                        ggTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACAt                   >  2:229297/1‑81 (MQ=255)                                                            gAAGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGt                <  2:229260/80‑1 (MQ=255)                                                             aaGATGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTc               <  2:229261/80‑1 (MQ=255)                                                                aTGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTg           >  2:229315/1‑81 (MQ=255)                                                                aTGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTg           >  2:229314/1‑81 (MQ=255)                                                                aTGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTg           >  2:229304/1‑81 (MQ=255)                                                                aTGAAATCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTg           >  1:229310/1‑81 (MQ=255)                                                                   aaaTCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGt         <  2:229262/80‑1 (MQ=255)                                                                   aaaTCATCCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTg        <  1:229263/81‑1 (MQ=255)                                                                       catcCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACt    >  2:229311/1‑81 (MQ=255)                                                                         tcCTCGTCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGc  <  2:229264/81‑1 (MQ=255)                                                                               |                                                                           AAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGC  >  NC_000913/1147104‑1147256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑