Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,161,670	A→G	intergenic (‑119/‑215)	fhuE ← / → hinT	ferric coprogen/ferric rhodotorulic acid outer membrane transporter/purine nucleoside phosphoramidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,161,670	0	A	G	100.0%	100.2 / NA	28	intergenic (‑119/‑215)	fhuE/hinT	ferric coprogen/ferric rhodotorulic acid outer membrane transporter/purine nucleoside phosphoramidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/11);  total (17/11)

GGGTGATGAGCGATTATATTTAGTGTGCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATATTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAGCGCATTCGCAGGCAGAAATGGGAATAATGGCGATATAT  >  NC_000913/1161597‑1161744                                                                          |                                                                           gggTGATGAGCGATTATATTTAGTGTGCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCa                                                                     >  1:231659/1‑81 (MQ=255)    tgatgaGCGATTATATTTAGTGTGCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGt                                                                  <  1:231630/81‑1 (MQ=255)       tgaGCGATTATATTTAGTGTGCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTaaa                                                               <  2:231631/81‑1 (MQ=255)        gaGCGATTATATTTAGTGTGCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAAc                                                              >  2:231649/1‑81 (MQ=255)          gCGATTATATTTAGTGTGCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAAcc                                                             >  1:231664/1‑80 (MQ=255)            gATTATATTTAGTGTGCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCaaa                                                          <  2:231632/81‑1 (MQ=255)                atatTTAGTGTGCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACa                                                        >  2:231656/1‑79 (MQ=255)                atatTTAGTGTGCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACa                                                        >  1:231651/1‑79 (MQ=255)                  atTTAGTGTGCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACagag                                                     >  1:231658/1‑80 (MQ=255)                   tttAGTGTGCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGaa                                                   >  2:231662/1‑81 (MQ=255)                       gtgtGCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGt                                                <  2:231633/80‑1 (MQ=255)                       gtgtGCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTc                                               >  1:231652/1‑81 (MQ=255)                         gtgCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCtt                                             >  2:231655/1‑81 (MQ=255)                          tgCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCttt                                            <  2:231634/81‑1 (MQ=255)                           gCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCtttt                                           >  2:231650/1‑81 (MQ=255)                               ataatTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAgc                                       <  2:231635/81‑1 (MQ=255)                                    tttgtttgTAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAGCGCAtt                                  >  2:231670/1‑81 (MQ=255)                                        tttgtAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAGCGCATTCg                                >  1:231660/1‑79 (MQ=255)                                         ttgtAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAGCGCATTCgcag                             >  2:231712/1‑81 (MQ=255)                                            tAAATGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAGCGCATTCgcaggc                           <  2:231636/80‑1 (MQ=255)                                              aaTGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAGCGCATTCgcaggcag                         >  2:231645/1‑80 (MQ=255)                                                tGCAAATGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAGCGCATTCGCAGGCAGaaa                      <  1:231637/81‑1 (MQ=255)                                                   aaaTGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAGCGCATTCGCAGGCAGAAATgg                   >  2:231654/1‑81 (MQ=255)                                                     aTGAGAAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAGCGCATTCGCAGGCAGAAATGGGa                 <  1:231638/81‑1 (MQ=255)                                                        agaAATATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAGCGCATTCGCAGGCAGAAATGGGaata              <  1:231639/81‑1 (MQ=255)                                                          aaaTATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAGCGCATTCGCAGGCAGAAATGGGaataat            >  1:231666/1‑81 (MQ=255)                                                           aaTATACGCATTCATGTTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAGCGCATTCGCAGGCAGAAATGGGAATAATg           >  1:231665/1‑81 (MQ=255)                                                                    aTTCATGTTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAGCGCATTCGCAGGCAGAAATGGGAATAATGGCGatatat  <  1:231640/81‑1 (MQ=255)                                                                          |                                                                           GGGTGATGAGCGATTATATTTAGTGTGCGAATAATTTTGTTTGTAAATGCAAATGAGAAATATACGCATTCATATTTGTCATGTAAACCAAACAGAGAATGTCTTTTCAGCGCATTCGCAGGCAGAAATGGGAATAATGGCGATATAT  >  NC_000913/1161597‑1161744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑