Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,177,323	G→A	A2T (GCG→ACG)	lolE →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,177,323	0	G	A	100.0%	103.4 / NA	29	A2T (GCG→ACG)	lolE	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/17);  total (12/17)

GAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCC  >  NC_000913/1177245‑1177400                                                                               |                                                                              gAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACg                                                                             <  2:234219/81‑1 (MQ=255)  aaaCGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGa                                                                            >  1:234254/1‑81 (MQ=255)    aCGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATg                                                                          <  2:234220/81‑1 (MQ=255)      gTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATGcc                                                                        <  1:234221/81‑1 (MQ=255)        aTGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGACGGGGGCGGAGTAATGACGATGCCtt                                                                      >  1:234259/1‑81 (MQ=255)           aGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTAt                                                                   >  1:234270/1‑81 (MQ=255)               gccAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGtt                                                               <  1:234222/81‑1 (MQ=255)               gccAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGtt                                                               <  1:234223/81‑1 (MQ=255)                   gTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGttatta                                                           <  1:234224/81‑1 (MQ=255)                         aaTGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGc                                                     >  2:234262/1‑81 (MQ=255)                              gTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCg                                                >  1:234260/1‑81 (MQ=255)                                gATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGtt                                              >  1:234255/1‑81 (MQ=255)                                 aTGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGtt                                              <  1:234225/80‑1 (MQ=255)                                       gtcTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTcg                                       <  2:234227/81‑1 (MQ=255)                                       gtcTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTc                                        <  1:234226/80‑1 (MQ=255)                                        tcTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTcgc                                      >  1:234266/1‑81 (MQ=255)                                        tcTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTcgc                                      <  1:234228/81‑1 (MQ=255)                                            aCGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGAc                                  <  2:234229/81‑1 (MQ=255)                                               gcggAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGAcggc                               >  1:234274/1‑81 (MQ=255)                                                  gAACTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACggcggc                            >  2:234273/1‑81 (MQ=255)                                                   aaCTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGgcggcg                           >  1:234267/1‑81 (MQ=255)                                                    aCTGAGCCTGATGGGGGCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGgcggcgc                          <  1:234230/81‑1 (MQ=255)                                                                 ggggCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGgtgt             <  2:234232/81‑1 (MQ=255)                                                                 ggggCGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGgtg              <  1:234231/80‑1 (MQ=255)                                                                     cGGAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCt         <  1:234233/81‑1 (MQ=255)                                                                      ggAGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCt         >  2:234279/1‑80 (MQ=255)                                                                        aGTAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGAt      >  2:234261/1‑81 (MQ=255)                                                                          tAATGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGAtct    <  1:234234/81‑1 (MQ=255)                                                                            aTGACGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTcc  <  2:234235/81‑1 (MQ=255)                                                                               |                                                                              GAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCC  >  NC_000913/1177245‑1177400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑