Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	160,809	A→T	L226L (CTT→CTA)	sfsA ←	putative DNA‑binding transcriptional regulator of maltose metabolism

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	160,809	0	A	T	100.0%	86.1 / NA	24	L226L (CTT→CTA)	sfsA	putative DNA‑binding transcriptional regulator of maltose metabolism
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (15/9);  total (15/9)

GTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTG  >  NC_000913/160734‑160879                                                                            |                                                                       gTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTTAGAGc                                                                   >  1:36969/1‑81 (MQ=255)  tATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTTAGAGcc                                                                  <  2:36941/81‑1 (MQ=255)   aTTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTATAATGTAACCGGCAGTGATTTTTTTAGAGCCa                                                                 >  2:36972/1‑81 (MQ=255)    tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTTAGAGCCa                                                                 <  1:36943/80‑1 (MQ=255)    tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTTAGAGCCAt                                                                <  2:36942/81‑1 (MQ=255)          cACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCAGCAGTGATTTTTTTAGAGCCATGCCTTc                                                          <  1:36944/81‑1 (MQ=255)            cgcgACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTTAGAGCCATGCCTTcag                                                        >  2:36973/1‑81 (MQ=255)             gcgACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTTAGAGCCATGCCTTcag                                                        >  1:36976/1‑80 (MQ=255)               gACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTTAGAGCCATGCCTTcag                                                        >  1:36964/1‑78 (MQ=255)                   aGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTTAGAGCCATGCCTTCAGCAGAAAt                                                 >  1:36989/1‑81 (MQ=255)                          aaaTTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTTAGAGCCATGCCTTCAGCAGAAATTTCCGCt                                          >  1:36990/1‑81 (MQ=255)                             ttAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTTAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTg                                       <  2:36945/81‑1 (MQ=255)                               aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTTAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGt                                      <  1:36946/80‑1 (MQ=255)                                      tacttactTTACTACAATGTAACCGGCAGTGATTTTTTTAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAg                               >  1:36985/1‑80 (MQ=255)                                       acttacttTACTACAATGTAACCGGCAGTGATTTTTTTAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGaa                             >  2:36971/1‑81 (MQ=255)                                       acttacttTACTACAATGTAACCGGCAGTGATTTTTTTAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGaa                             >  2:36979/1‑81 (MQ=255)                                        cttacttTACTACAATGTAACCGGCAGTGATTTTTTTAGAGCGACGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAt                            >  1:36981/1‑81 (MQ=255)                                          tactttactACAATGTAACCGGCAGTGATTTTTTTAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAttt                          >  1:36974/1‑81 (MQ=255)                                           actttactACAATGTAACCGGCAGTGATTTTTTTAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTc                         >  1:36980/1‑81 (MQ=255)                                               tactacAATGTAACCGGCAGTGATTTTTTTAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAc                      <  1:36947/80‑1 (MQ=255)                                                        gTAACCGGCAGTGATTTTTTTAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGt            >  1:36983/1‑81 (MQ=255)                                                           aCCGGCAGTGATTTTTTTAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTg          <  2:36948/80‑1 (MQ=255)                                                                  gTGATTTTTTTAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCt   >  2:36977/1‑80 (MQ=255)                                                                  gTGATTTTTTTAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTg  <  1:36949/81‑1 (MQ=255)                                                                            |                                                                       GTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTG  >  NC_000913/160734‑160879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑