Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,210,465	C→T	Y40Y (TAC→TAT)	mcrA →	e14 prophage; 5‑methylcytosine‑specific restriction enzyme McrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,210,465	0	C	T	100.0%	41.9 / NA	13	Y40Y (TAC→TAT)	mcrA	e14 prophage; 5‑methylcytosine‑specific restriction enzyme McrA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/5);  total (8/5)

TGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCT  >  NC_000913/1210388‑1210533                                                                              |                                                                     tgtTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTATAat                                                                   <  2:239185/81‑1 (MQ=255)       aTAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTATAATATCGct                                                             <  1:239186/81‑1 (MQ=255)         aGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTATAATATCGctct                                                           >  1:239209/1‑81 (MQ=255)                           ttATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTATAATATCGCTCTTGATTATATCATTGAACg                                         >  1:239204/1‑81 (MQ=255)                                gTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTATAATATCGCTCTTGATTATATCATTGAACggttgg                                    <  1:239187/81‑1 (MQ=255)                                     aTCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTATAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGAt                               >  1:239205/1‑81 (MQ=255)                                                gTGGGGGCCGGGTGACAGAAACAAAGATTATAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATc                    <  1:239188/81‑1 (MQ=255)                                                    gggCCGGGTGACAGAAACAAAGATTATAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCaa                <  1:239189/81‑1 (MQ=255)                                                           gTGACAGAAACAAAGATTATAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCAAGTCgtag         >  2:239212/1‑81 (MQ=255)                                                             gACAGAAACAAAGATTATAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCAAGTCgtagta       >  1:239210/1‑81 (MQ=255)                                                             gACAGAAACAAAGATTATAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCAAGTCgtagta       >  2:239211/1‑81 (MQ=255)                                                              aCAGAAACAAAGATTATAATATCGCTCTTGATTATATTTTTGAACGGTTGGTTGATTCCGGTGTATCCCAAGTCgtagta       >  1:239207/1‑80 (MQ=255)                                                                  aaaCAAAGATTATAATATCGCTCTTGATTATATCATTGAACGGTCGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCt  >  1:239206/1‑81 (MQ=255)                                                                              |                                                                     TGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCT  >  NC_000913/1210388‑1210533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑