Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,256,202	T→C	intergenic (‑250/+519)	ycgV ← / ← ychF	putative autotransporter adhesin YcgV/redox‑responsive ATPase YchF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,256,202	0	T	C	100.0%	27.3 / NA	9	intergenic (‑250/+519)	ycgV/ychF	putative autotransporter adhesin YcgV/redox‑responsive ATPase YchF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/3);  total (6/3)

CCCCTTACAAAGGTGAGTTTATATCGAGTTATTCCCTAAGTTGTCGTTACTTATTCCAATTCGTTACTATAAATATGGCAAAAATATTACAACAAACAGCGGTTAAAAAATTTGGTGAAGATTTTTTGTAATAATCC  >  NC_000913/1256135‑1256271                                                                    |                                                                      ccccTTACAAAGGTGAGTTTATATCGAGTTATTCCCTAAGTTGTCGTTACTTATTCCAATTCGTTACCATAAATATGGCa                                                           <  1:245233/80‑1 (MQ=255)     ttACAAAGGTGAGTTTATATCGAGTTATTCCCTAAGTTGTCGTTACTTATTCCAATTCGTTACCATAAATATGGCaaaaa                                                       >  2:245243/1‑80 (MQ=255)      tACAAAGGTGAGTTTATATCGAGTTATTCCCTAAGTTGTCGTTACTTATTCCAATTCGTTACCATAAATATGGCAAAAata                                                     >  2:245247/1‑81 (MQ=255)              tGAGTTTATATCGAGTTATTCCCTAAGTTGTCGTTACTTATTCCAATTCGTTACCATAAATATGGCAAAAATATTacaac                                              >  2:245246/1‑80 (MQ=255)                      tatCGAGTTATTCCCTAAGTTGTCGTTACTTATTCCAATTCGTTACCATAAATATGGCAAAAATATTACAACAAACAGCgg                                     >  1:245254/1‑81 (MQ=255)                                ttCCCTAAGTTGTCGTTACTTATTCCAATTCGTTACCATAAATATGGCAAAAATATTACAACAAACAGCGGTTAAAAAAt                            <  2:245234/80‑1 (MQ=255)                                           gTCGTTACTTATTCCAATTCGTTACCATAAATATGGCAAAAATATTACAACAAACAGCGGTTAAAAAATTTGGTGAAGAt                 <  2:245235/80‑1 (MQ=255)                                                     aTTCCAATTCGTTACCATAAATATGGCAAAAATATTACAACAAACAGCGGTTAAAAAATTTGGTGAAGATTTTTTGtaat       >  1:245249/1‑80 (MQ=255)                                                         cAATTCGTTACCATAAATATGGCAAAAATATTACAACAAACAGCGGTTAAAAAATTTGGTGAAGATTTTTTGTAATAATcc  >  1:245250/1‑81 (MQ=255)                                                                    |                                                                      CCCCTTACAAAGGTGAGTTTATATCGAGTTATTCCCTAAGTTGTCGTTACTTATTCCAATTCGTTACTATAAATATGGCAAAAATATTACAACAAACAGCGGTTAAAAAATTTGGTGAAGATTTTTTGTAATAATCC  >  NC_000913/1256135‑1256271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑