Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,265,511	G→A	S167N (AGC→AAC)	prfA →	peptide chain release factor RF1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,265,511	0	G	A	100.0%	116.8 / NA	33	S167N (AGC→AAC)	prfA	peptide chain release factor RF1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/12);  total (21/12)

CCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG  >  NC_000913/1265433‑1265587                                                                               |                                                                             cccGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACg                                                                            >  2:246715/1‑81 (MQ=255)   cgccgcTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGt                                                                          >  2:246724/1‑81 (MQ=255)         tGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGgt                                                                    >  2:246725/1‑81 (MQ=255)          ggCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGgtg                                                                   >  2:246732/1‑81 (MQ=255)                tAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATg                                                             >  2:246712/1‑81 (MQ=255)                 aGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATgg                                                            >  2:246729/1‑81 (MQ=255)                   aaaTCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGgtc                                                          <  1:246691/81‑1 (MQ=255)                      tCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGgtcgtc                                                       >  1:246721/1‑81 (MQ=255)                        aTGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCt                                                      <  2:246692/80‑1 (MQ=255)                          gAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGa                                                    >  2:246748/1‑80 (MQ=255)                          gAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGa                                                    >  1:246728/1‑80 (MQ=255)                           aGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGaa                                                   <  2:246693/80‑1 (MQ=255)                             cgcCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAAtt                                                <  1:246694/81‑1 (MQ=255)                                 aGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGaa                                            >  1:246736/1‑81 (MQ=255)                                  gCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAAt                                           >  2:246761/1‑81 (MQ=255)                                     aGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCcg                                        >  1:246722/1‑81 (MQ=255)                                     aGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCcg                                        >  1:246737/1‑81 (MQ=255)                                      gggTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCcgg                                       >  2:246733/1‑81 (MQ=255)                                       ggTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCcggc                                      >  2:246741/1‑81 (MQ=255)                                         tGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCcggcgg                                    <  2:246695/81‑1 (MQ=255)                                          gAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGt                                   >  2:246734/1‑81 (MQ=255)                                             cATGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCAt                                <  1:246696/81‑1 (MQ=255)                                              aTGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATc                               <  1:246698/81‑1 (MQ=255)                                              aTGGTGGTTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATc                               <  2:246697/81‑1 (MQ=255)                                                    gtTATAAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGc                         <  2:246699/81‑1 (MQ=255)                                                        taAAGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACg                     >  1:246720/1‑81 (MQ=255)                                                         aaaGAGATCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACgt                    >  1:246746/1‑81 (MQ=255)                                                            gagaTCATCGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGtt                 >  1:246730/1‑81 (MQ=255)                                                                tcatcGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTg             >  2:246717/1‑81 (MQ=255)                                                                  atcGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCt           <  1:246700/81‑1 (MQ=255)                                                                   tcGCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTa          <  2:246701/81‑1 (MQ=255)                                                                     gCCAAAATTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACg        >  2:246747/1‑81 (MQ=255)                                                                           aTTAACGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCg  <  2:246702/81‑1 (MQ=255)                                                                               |                                                                             CCCGCCGCTGGCGGGTAGAAATCATGAGCGCCAGCGAGGGTGAACATGGTGGTTATAAAGAGATCATCGCCAAAATTAGCGGTGATGGTGTGTATGGTCGTCTGAAATTTGAATCCGGCGGTCATCGCGTGCAACGTGTTCCTGCTACGGAATCG  >  NC_000913/1265433‑1265587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑