Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,277,223	G→C	A132A (GCC→GCG)	narX ←	sensory histidine kinase NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,277,223	0	G	C	100.0%	89.6 / NA	26	A132A (GCC→GCG)	narX	sensory histidine kinase NarX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (12/14);  total (12/14)

CCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATT  >  NC_000913/1277147‑1277297                                                                             |                                                                           cccGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGcaa                                                                        >  2:248530/1‑81 (MQ=255)   cGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGcaaca                                                                      <  2:248499/81‑1 (MQ=255)         aCCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTgg                                                                >  1:248526/1‑81 (MQ=255)            aGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTg                                                             <  2:248500/81‑1 (MQ=255)               aCCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACa                                                          >  2:248524/1‑81 (MQ=255)                ccACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACAt                                                         <  2:248501/81‑1 (MQ=255)                     gTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCt                                                    >  1:248542/1‑81 (MQ=255)                          gATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGacac                                               >  2:248540/1‑81 (MQ=255)                             gcgcATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGt                                            >  1:248534/1‑81 (MQ=255)                             gcgcATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGt                                            >  1:248537/1‑81 (MQ=255)                               gcATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGttt                                          <  1:248502/81‑1 (MQ=255)                                 aTTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTtct                                        >  2:248532/1‑81 (MQ=255)                                  tttCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTtcta                                       <  1:248503/81‑2 (MQ=255)                                    tCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCg                                      <  1:248504/80‑1 (MQ=255)                                       gtggtgCGGTCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGttt                                  >  2:248529/1‑81 (MQ=255)                                                tCAAAACCAGATACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACg                          >  2:248544/1‑80 (MQ=255)                                                           tACCAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAgg              <  1:248505/81‑1 (MQ=255)                                                             ccAGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAggg             <  2:248506/80‑1 (MQ=255)                                                               aGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATc          <  1:248509/81‑1 (MQ=255)                                                               aGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATc          <  1:248508/81‑1 (MQ=255)                                                               aGTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATc          <  1:248507/81‑1 (MQ=255)                                                                gTTGATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGAt           >  2:248536/1‑79 (MQ=255)                                                                   gATCAAGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGtt      >  2:248535/1‑81 (MQ=255)                                                                       aaGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCAtt  <  1:248511/81‑1 (MQ=255)                                                                       aaGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCAtt  <  1:248510/81‑1 (MQ=255)                                                                       aaGCCCCGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCACAGGGATCAGTTCAtt  <  1:248512/81‑1 (MQ=255)                                                                             |                                                                           CCCGATGGACCAGTACCACTGTCTCGATGCGCATTTCCGTGGTGCGGTCAAAACCAGATACCAGTTGATCAAGCCCGGCAACAAACTGGCTGACATCCGCTGACACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATT  >  NC_000913/1277147‑1277297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑