Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,284,681	T→C	S360P (TCT→CCT)	narH →	nitrate reductase A subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,284,681	0	T	C	100.0%	87.2 / NA	25	S360P (TCT→CCT)	narH	nitrate reductase A subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/7);  total (18/7)

AATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTA  >  NC_000913/1284605‑1284756                                                                             |                                                                            aatggaatggAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTcc                                                                         <  2:249755/81‑1 (MQ=255)  atggaatggaAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTcc                                                                         >  1:249781/1‑80 (MQ=255)          gaaACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTc                                                                <  2:249756/81‑1 (MQ=255)           aaaCTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCt                                                               <  1:249757/81‑1 (MQ=255)               tGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTgcag                                                           >  2:249780/1‑81 (MQ=255)                ggCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTgcag                                                           >  1:249790/1‑80 (MQ=255)                ggCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTgcag                                                           >  2:249795/1‑80 (MQ=255)                  cgctgccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTgcag                                                           >  2:249800/1‑78 (MQ=255)                  cgctgccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTgcag                                                           >  1:249785/1‑78 (MQ=255)                     tgccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGAcg                                                     >  2:249789/1‑81 (MQ=255)                      gccgctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGAcgc                                                    >  2:249799/1‑81 (MQ=255)                         gctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCggg                                                 >  1:249793/1‑81 (MQ=255)                          ctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGt                                                >  1:249803/1‑81 (MQ=255)                          ctgcATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGt                                                <  2:249758/81‑1 (MQ=255)                             cATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGa                                              <  1:249759/80‑1 (MQ=255)                                   gAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTggg                                        >  1:249796/1‑80 (MQ=255)                                       atCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGc                                    >  1:249802/1‑80 (MQ=255)                                                    gATGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGc                       >  2:249804/1‑80 (MQ=255)                                                      tGGTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGc                       >  1:249809/1‑78 (MQ=255)                                                        gTCTGGTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGAc                  >  2:249808/1‑81 (MQ=255)                                                             gTACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCg              <  1:249760/80‑1 (MQ=255)                                                              tACGTGCCGCCTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGa             >  2:249817/1‑80 (MQ=255)                                                                   gccgccTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCt       >  1:249806/1‑81 (MQ=255)                                                                     cgccTCTGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGc     <  2:249761/81‑1 (MQ=255)                                                                        ctctGCCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTa  >  2:249811/1‑81 (MQ=255)                                                                             |                                                                            AATGGAATGGAAACTGGCGCTGCCGCTGCATCCGGAATATCGCACACTGCCGATGGTCTGGTACGTGCCGCCTCTGTCTCCGATTCAGTCTGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTA  >  NC_000913/1284605‑1284756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑