Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,062	G→A	intergenic (+78/+4)	ychS → / ← rttR	putative uncharacterized protein YchS/small RNA RttR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,062	0	G	A	100.0%	27.3 / NA	10	intergenic (+78/+4)	ychS/rttR	putative uncharacterized protein YchS/small RNA RttR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/5);  total (5/5)

AGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAC  >  NC_000913/1286989‑1287130                                                                          |                                                                     aGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAATGAtggt                                                               <  1:250207/81‑1 (MQ=255)            cTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAATGATGGTGGTGGGGGAAg                                                    >  1:250232/1‑81 (MQ=255)               ccccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAATGATGGTGGTGGGGGAAGGAt                                                 >  2:250227/1‑81 (MQ=255)                      tGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAATGATGGTGGTGGGGGAAGGATTACTCAg                                          <  2:250208/81‑1 (MQ=255)                                        gCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAATGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCt                        >  1:250233/1‑81 (MQ=255)                                            aTTCGCTTGAGAGTTCAGGGACTTTTGAAAATGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCgg                    >  2:250231/1‑81 (MQ=255)                                                     agagTTCAGGGACTTTTGAAAATGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGcc           >  2:250239/1‑81 (MQ=255)                                                           cAGGGACTTTTGAAAATGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTACgg      <  2:250209/80‑1 (MQ=255)                                                            aGGGACTTTTGAAAATGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCa    <  2:250210/81‑1 (MQ=25)                                                              ggACTTTTGAAAATGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAc  <  2:250211/81‑1 (MQ=18)                                                                          |                                                                     AGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAC  >  NC_000913/1286989‑1287130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑