Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,290,932	C→T	L231F (CTT→TTT)	rssB →	regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,290,932	0	C	T	100.0%	78.1 / NA	23	L231F (CTT→TTT)	rssB	regulator of RpoS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/14);  total (9/14)

ACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCC  >  NC_000913/1290857‑1291006                                                                            |                                                                            aCCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGTTTGc                                                                         <  2:250710/80‑1 (MQ=255)           gACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGTTTGCACACC‑AAAATc                                                             <  2:250711/81‑1 (MQ=255)               tAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGTTTGCACACC‑AAAATCAACg                                                         >  2:250742/1‑81 (MQ=255)                 aTGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGTTTGCACACC‑AAAATCAACGGt                                                       >  1:250741/1‑81 (MQ=255)                       tACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGTTTGCACACC‑AAAATCAACGGTTGCCag                                                 <  2:250712/81‑1 (MQ=255)                       tACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGTTTGCACACC‑AAAATCAACGGTTGCCag                                                 <  2:250713/81‑1 (MQ=255)                          ttGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGt                                              >  1:250743/1‑81 (MQ=255)                           tgctgcCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGtt                                             >  2:250744/1‑81 (MQ=255)                                 cTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGgcgc                                       >  2:250740/1‑81 (MQ=255)                                     ttattaCGCGCATTGTTTAACGGATTATTACAGGAACAGTTTGCACACCAAAAATCAACGGTTGCCAGAGTTGGGCGCGtt                                    <  2:250714/81‑1 (MQ=255)                                        ttaCGCGCATTGTTTAACGGATTATTACAGGAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGGCGCGTTAtt                                 <  2:250715/80‑1 (MQ=255)                                         taCGCGCATTGTTTAACGGATTATTACAGGAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTg                                >  1:250739/1‑80 (MQ=255)                                           cgcgCATTGTTTAACGGATTATTACAGGAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAg                             <  2:250716/81‑1 (MQ=255)                                            gcgcATTGTTTAACGGATTATTACAGGAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAg                             >  2:250737/1‑80 (MQ=255)                                                     ttAACGGATTATTACAGGAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAAc                    >  2:250738/1‑80 (MQ=255)                                                         cGGATTATTACAGGAAGAGTTTGCACACC‑AAAATAAACGGTTGCCAGAGTTGGGCGAGTTATTGAAGCAGGTAAACCAttg               <  2:250717/81‑2 (MQ=255)                                                               attaCAGGAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTc          <  1:250718/80‑1 (MQ=255)                                                               attaCAGGAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCg         >  2:250732/1‑81 (MQ=255)                                                                ttaCAGGAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCg         <  2:250720/80‑1 (MQ=255)                                                                ttaCAGGAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCg         <  1:250719/80‑1 (MQ=255)                                                                     ggAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGc   <  2:250721/81‑1 (MQ=255)                                                                      gAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGcc  <  2:250722/81‑1 (MQ=255)                                                                      gAACAGTTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGcc  <  1:250723/81‑1 (MQ=255)                                                                            |                                                                            ACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACC‑AAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCC  >  NC_000913/1290857‑1291006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑