Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,298,931	A→G	T112A (ACC→GCC)	ychE →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,298,931	0	A	G	100.0%	66.0 / NA	19	T112A (ACC→GCC)	ychE	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/7);  total (12/7)

TGACAATAGCGATGTCGATGATCAGCGGCAAGCTTGGCGAGGATAAACAGAACAAGCAAGAAAAATCAGAAACCGCGGTACGTGAAAGCATTGGTGTGGTGCCACTGGCGTTGCCGTTGATGGCGGGGCCAGGGGCGATCAGTTC  >  NC_000913/1298860‑1299004                                                                        |                                                                          tgACAATAGCGATGTCGATGATCAGCGGCAAGCTTGGCGAGGATAAACAGAACAAGCAAGAAAAATCAGAAGCCGCGGTAc                                                                  >  2:251888/1‑81 (MQ=255)      aTAGCGATGTCGATGATCAGCGGCAAGCTTGGCGAGGATAAACAGAACAAGCAAGAAAAATCAGAAGCCGCGGTACGTGaa                                                             <  1:251864/81‑1 (MQ=255)           gATGTCGATGATCAGCGGCAAGCTTGGCGAGGATAAACAGAACAAGCAAGAAAAATCAGAAGCCGCGGTACGTGAAAGCAt                                                        >  1:251895/1‑81 (MQ=255)            aTGTCGATGATCAGCGGCAAGCTTGGCGAGGATAAACAGAACAAGCAAGAAAAATCAGAAGCCGCGGTACGTGAAAGCAtt                                                       <  2:251865/81‑1 (MQ=255)                 gatgatCAGCGGCAAGCTTGGCGAGGATAAACAGAACAAGCAAGAAAAATCAGAAGCCGCGGTACGTGAAAGCATtggtgt                                                  >  1:251881/1‑81 (MQ=255)                 gatgatCAGCGGCAAGCTTGGCGAGGATAAACAGAACAAGCAAGAAAAATCAGAAGCCGCGGTACGTGAAAGCATtggtgt                                                  >  1:251886/1‑81 (MQ=255)                  atgatCAGCGGCAAGCTTGGCGAGGATAAACAGAACAAGCAAGAAAAATCAGAAGCCGCGGTACGTGAAAGCATtggtgtg                                                 >  1:251894/1‑81 (MQ=255)                         gCGGCAAGCTTGGCGAGGATAAACAGAACAAGCAAGAAAAATCAGAAGCCGCGGTACGTGAAAGCATTGGTGTGGTGCCAc                                          <  2:251866/81‑1 (MQ=255)                           ggCAAGCTTGGCGAGGATAAACAGAACAAGCAAGAAAAATCAGAAGCCGCGGTACGTGAAAGCATTGGTGTGGTGCCACTg                                        <  1:251867/81‑1 (MQ=255)                                 cTTGGCGAGGATAAACAGAACAAGCAAGAAAAATCAGAAGCCGCGGTACGTGAAAGCATTGGTGTGGTGCCACTGGCGTTg                                  >  2:251892/1‑81 (MQ=255)                                           aTAAACAGAACAAGCAAGAAAAATCAGAAGCCGCGGTACGTGAAAGCATTGGTGTGGTGCCACTGGCGTTGCCGTTGATgg                        >  2:251915/1‑81 (MQ=255)                                              aaCAGAACAAGCAAGAAAAATCAGAAGCCGCGGTACGTGAAAGCATTGGTGTGGTGCCACTGGCGTTGCCGTTGATGGCgg                     >  1:251890/1‑81 (MQ=255)                                                 aGAACAAGCAAGAAAAATCAGAAGCCGCGGTACGTGAAAGCATTGGTGTGGTGCCACTGGCGTTGCCGTTGATGGCGGGGc                  >  1:251899/1‑81 (MQ=255)                                                  gAACAAGCAAGAAAAATCAGAAGCCGCGGTACGTGAAAGCATTGGTGTGGTGCCACTGGCGTTGCCGTTGATGGCGGGGcc                 <  1:251868/81‑1 (MQ=255)                                                   aaCAAGCAAGAAAAATCAGAAGCCGCGGTACGTGAAAGCATTGGTGTGGTGCCACTGGCGTTGCCGTTGATGGCGGGGc                  >  1:251889/1‑79 (MQ=255)                                                       agcaagAAAAATCAGAAGCCGCGGTACGTGAAAGCATTGGTGTGGTGCCACTGGCGTTGCCGTTGATGGCGGGGCCAgggg            <  1:251869/81‑1 (MQ=255)                                                         caagAAAAATCAGAAGCCGCGGTACGTGAAAGCATTGGTGTGGTGCCACTGGCGTTGCCGTTGATGGCGGGGCCAGGGGCg          <  1:251870/81‑1 (MQ=255)                                                                aaTCAGAAGCCGCGGTACGTGAAAGCATTGGTGTGGTGCCACTGGCGTTGCCGTTGATCGCGGGGCCAGGGGCGATCAGtt   >  1:251901/1‑81 (MQ=255)                                                                  tCAGAAGCCGCGGTACGTGAAAGCATTGGTGTGGTGCCACTGGCGTTGCCGTTGATGGCGGGGCCAGGGGCGATCAGTTc  >  1:251896/1‑80 (MQ=255)                                                                        |                                                                          TGACAATAGCGATGTCGATGATCAGCGGCAAGCTTGGCGAGGATAAACAGAACAAGCAAGAAAAATCAGAAACCGCGGTACGTGAAAGCATTGGTGTGGTGCCACTGGCGTTGCCGTTGATGGCGGGGCCAGGGGCGATCAGTTC  >  NC_000913/1298860‑1299004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑