Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,299,534	T→C	F2L (TTT→CTT)	insH21 →	IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,299,534	0	T	C	100.0%	32.8 / NA	11	F2L (TTT→CTT)	insH21	IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/1);  total (10/1)

ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGT  >  NC_000913/1299460‑1299572                                                                           |                                       attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCa                                  >  1:251955/1‑81 (MQ=255)      taATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTg                              >  2:251997/1‑80 (MQ=255)          tCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGc                          >  1:251950/1‑80 (MQ=255)          tCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGc                          >  2:251945/1‑80 (MQ=255)           ccACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCa                        >  2:251948/1‑81 (MQ=255)                   tttGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAg                >  2:251943/1‑81 (MQ=255)                     tGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAggg              >  1:251946/1‑81 (MQ=255)                        tAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGTtc           <  1:251924/81‑1 (MQ=255)                           agGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGTtcat         >  1:251944/1‑80 (MQ=255)                           agGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGTtcat         >  2:251963/1‑80 (MQ=255)                                  tGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGt  >  2:251966/1‑80 (MQ=14)                                                                           |                                       ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGT  >  NC_000913/1299460‑1299572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑