Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,309,276	C→A	A332S (GCA→TCA)	kch ←	K(+) channel Kch

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,309,276	0	C	A	100.0%	62.3 / NA	18	A332S (GCA→TCA)	kch	K(+) channel Kch
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/9);  total (9/9)

TATTCAGGTTTTTGCTATCACTGACGGCGAGAACTGTTTTGACATCACTGCTCATATCTTTTGCCGAGAGTACAACAAACGCGTTATCTGCATCGTTATCACTCAGCGCCAGAATGGCCCGGCAACGATCGAT  >  NC_000913/1309213‑1309345                                                                |                                                                      taTTCAGGTTTTTGCTATCACTGACGGCGAGAACTGTTTTGACATCACTGCTCATATCTTTTGACGAGAGTACAACAAAcg                                                      >  2:253424/1‑81 (MQ=255)   ttCAGGTTTTTGCTATCACTGACGGCGAGAACTGTTTTGACATCACTGCTCATATCTTTTGACGAGAGTACAACAAAcgcg                                                    >  1:253426/1‑81 (MQ=255)      aGGTTTTTGCTATCACTGACGGCGAGAACTGTTTTGACATCACTGCTCATATCTTTTGACGAGAGTACAACAAACGCGtt                                                  <  2:253408/80‑1 (MQ=255)              gCTATCACTGACGGCGAGAACTGTTTTGACATCACTGCTCATATCTTTTGACGAGAGTACAACAAACGCGTTATCTGCATc                                         >  2:253433/1‑81 (MQ=255)                  tCACTGACGGCGAGAACTGTTTTGACATCACTGCTCATATCTTTTGACGAGAGTACAACAAACGCGTTATCTGCATCGtt                                      <  1:253409/80‑1 (MQ=255)                   cACTGACGGCGAGAACTGTTTTGACATCACTGCTCATATCTTTTGACGAGAGTACAACAAACGCGTTATCTGCATCGTTAt                                    >  2:253429/1‑81 (MQ=255)                          ggCGAGAACTGTTTTGACATCACTGCTCATATCTTTTGACGAGAGTACAACAAACGCGTTATCTGCATCGTTATCACTCAg                             >  2:253430/1‑81 (MQ=255)                            cGAGAACTGTTTTGACATCACTGCTCATATCTTTTGACGAGAGTACAACAAACGCGTTATCTGCATCGTTATCACTCAgcg                           >  2:253428/1‑81 (MQ=255)                                    gTTTTGACATCACTGCTCATATCTTTTGACGAGAGTACAACAAACGCGTTATCTGCATCGTTATCACTCAGCGCCAGAATg                   >  1:253431/1‑81 (MQ=255)                                     ttttGACATCACTGCTCATATCTTTTGACGAGAGTACAACAAACGCGTTATCTGCATCGTTATCACTCAGCGCCAGAAt                    <  1:253410/79‑1 (MQ=255)                                     ttttGACATCACTGCTCATATCTTTTGACGAGAGTACAACAAACGCGTTATCTGCATCGTTATCACTCAGCGCCAGAATg                   <  2:253411/80‑1 (MQ=255)                                       ttGACATCACTGCTCATATCTTTTGACGAGAGTACAACAAACGCGTTATCTGCATCGTTATCACTCAGCGCCAGAATGGcc                >  1:253432/1‑81 (MQ=255)                                          aCATCACTGCTCATATCTTTT‑ACGAGAGTACAACAAACGCGTTATCTGCATCGTTATCACTCAGCGCCAGAATGGCCCGGc            <  1:253412/81‑1 (MQ=255)                                           cATCACTGCTCATATCTTTTGACGAGAGTACAACAAACGCGTTATCTGCATCGTTATCACTCAGCGCCAGAATGGCCCGGc            <  1:253413/81‑1 (MQ=255)                                               aCTGCTCATATCTTTTGACGAGAGTACAACAAACGCGTTATCTGCATCGTTATCACTCAGCGCCAGAATGGCCCGGCAAc         <  2:253414/80‑1 (MQ=255)                                                cTGCTCATATCTTTTGACGAGAGTACAACAAACGCGTTATCTGCATCGTTATCACTCAGCGCCAGAATGGCCCGGCAAcga       >  2:253434/1‑81 (MQ=255)                                                   cTCATATCTTTTGACGAGAGTACAACAAACGCGTTATCTGCATCGTTATCACTCAGCGCCAGAATGGCCCGGCAAcgatcg    <  1:253415/81‑1 (MQ=255)                                                      atatCTTTTGACGAGAGTACAACAAACGCGTTATCTGCATCGTTATCACTCAGCGCCAGAATGGCCCGGCAAcgatcgat  <  1:253416/80‑1 (MQ=255)                                                                |                                                                      TATTCAGGTTTTTGCTATCACTGACGGCGAGAACTGTTTTGACATCACTGCTCATATCTTTTGCCGAGAGTACAACAAACGCGTTATCTGCATCGTTATCACTCAGCGCCAGAATGGCCCGGCAACGATCGAT  >  NC_000913/1309213‑1309345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑