Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,333,565	G→A	V840I (GTC→ATC)	topA →	DNA topoisomerase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,333,565	0	G	A	100.0%	43.0 / NA	13	V840I (GTC→ATC)	topA	DNA topoisomerase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/10);  total (3/10)

GCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAA  >  NC_000913/1333490‑1333642                                                                            |                                                                              gCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATATctct                                                                          <  1:256887/81‑1 (MQ=255)     aTGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATATCTCTTCg                                                                       >  2:256910/1‑80 (MQ=255)            acaGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATATCTCTTCGGAAAAAGa                                                               <  1:256888/81‑1 (MQ=255)               gcagGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCTAACAGCAATATATCTCTTCGGAAAAAGACg                                                             >  2:256909/1‑80 (MQ=255)                       cGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATATCTCTTCGGAAAAAGACGGAAAGGCg                                                     <  1:256889/80‑1 (MQ=255)                               aataaGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATATCTCTTCGGAAAAAGACGGAAAGGCGActggctgg                                            >  1:256906/1‑81 (MQ=255)                                ataaGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATATCTCTTCGGAAAAAGACGGAAAGGCGActggctgg                                            <  2:256890/80‑1 (MQ=255)                                   aGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATATCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCa                                         <  1:256891/80‑1 (MQ=255)                                    gACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATATCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGc                                       <  2:256892/81‑1 (MQ=255)                                               gCTTTAGCCGTAAAACCAAACAGCAATATATCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGtt                             <  2:256893/80‑1 (MQ=255)                                                         tAAAACCAAACAGCAATATATCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATg                  <  1:256894/81‑1 (MQ=255)                                                               cAAACAGCAATATATCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGa            <  1:256895/81‑1 (MQ=255)                                                               cAAACAGCAATATATCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGa            <  1:256896/81‑1 (MQ=255)                                                                         tataTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGaaaaaaa  <  2:256898/81‑1 (MQ=255)                                                                            |                                                                              GCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAA  >  NC_000913/1333490‑1333642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑