Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,344,488	C→G	R41P (CGC→CCC)	yciZ ←	UPF0509 protein YciZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,344,488	0	C	G	100.0%	56.8 / NA	17	R41P (CGC→CCC)	yciZ	UPF0509 protein YciZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (10/7);  total (10/7)

ACCTGAAGGCACGATTGTTAAATCTCCCACGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGCGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCAGAGTGGTAATCGCCTGCCACAAGGAT  >  NC_000913/1344419‑1344555                                                                      |                                                                    aCCTGAAGGCACGATTGTTAAATCTCCCACGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGGGCAAGAGTTCt                                                          >  1:258473/1‑81 (MQ=255) aCCTGAAGGCACGATTGTTAAATCTCCCACGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGGGCAAGAGTTCt                                                          >  2:258468/1‑81 (MQ=255)      aaGGAACGATTGTTAAATCTCCCACGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGGGCAAGAGTTCTGCtt                                                      <  1:258452/80‑1 (MQ=255)                     aaTCTCCCACGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGGGCAAGAGTTCTGCTTTAAGTATCTCGAGAt                                       >  2:258472/1‑80 (MQ=255)                          cctaCGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGGGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGa                                 >  1:258484/4‑81 (MQ=255)                           ccACGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGGGCAAGAGTTCTGCTTTAAGTATCTCGAGATTAt                                    <  2:258453/77‑1 (MQ=255)                            cACGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGGGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATc                               >  2:258476/1‑81 (MQ=255)                                gTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGGGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCg                              >  1:258485/1‑78 (MQ=255)                                gTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGGGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCg                              >  2:258480/1‑78 (MQ=255)                                gTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGGGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCag                           <  1:258454/81‑1 (MQ=255)                                 tGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGGGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCaga                          <  2:258455/81‑1 (MQ=255)                                      tCGGGATGTCTGGCGTAGATTCAGCAACCTGGGGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCAGAGTgg                      >  2:258482/1‑80 (MQ=255)                                         ggATGTCTGGCGTAGATTCAGCAACCTGGGGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCAGAGTGGTAAt                  <  2:258456/81‑1 (MQ=255)                                                 ggCGTAGATTCAGCAACCTGGGGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCAGAGTGGTAATCGCCTGc           >  1:258481/1‑80 (MQ=255)                                                    gTAGATTCAGCAACCTGGGGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCAGAGTGGTAATCGCCTGCcaca       <  2:258457/81‑1 (MQ=255)                                                        aTTCAGCAACCTGGGGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCTGAGTGGTAATCGCCTGCCACAAGGa   >  2:258488/1‑81 (MQ=255)                                                         ttCAGCAACCTGGGGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCAGAGTGGTAATCGCCTGCCACAAGGat  <  2:258458/81‑1 (MQ=255)                                                                      |                                                                    ACCTGAAGGCACGATTGTTAAATCTCCCACGGTGCCTTCGGGATGTCTGGCGTAGATTCAGCAACCTGGCGCAAGAGTTCTGCTTTAAGTATCTCGAGATTATGGATCGCAGAGTGGTAATCGCCTGCCACAAGGAT  >  NC_000913/1344419‑1344555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑