Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,347,438	T→G	Y492S (TAC→TCC)	rnb ←	RNase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,347,438	0	T	G	100.0%	109.7 / NA	31	Y492S (TAC→TCC)	rnb	RNase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/13);  total (18/13)

TCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGG  >  NC_000913/1347360‑1347515                                                                               |                                                                              tCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGAt                                                                             >  2:258898/1‑81 (MQ=255) tCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGAt                                                                             >  2:258897/1‑81 (MQ=255)       ttGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTc                                                                        >  2:258879/1‑80 (MQ=255)          aTAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGa                                                                    >  1:258886/1‑81 (MQ=255)           tAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGAc                                                                   >  2:258882/1‑81 (MQ=255)              ccGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGAcc                                                                  >  1:258895/1‑79 (MQ=255)                gCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAgg                                                              <  2:258853/81‑1 (MQ=255)                         agACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGa                                                     <  2:258854/81‑1 (MQ=255)                            cGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGa                                                  >  1:258894/1‑81 (MQ=255)                             ggtggtTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGAc                                                 >  2:258906/1‑81 (MQ=255)                             ggtggtTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGAc                                                 <  1:258855/81‑1 (MQ=255)                              gtggtTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGAcc                                                <  1:258856/81‑1 (MQ=255)                               tggtTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCg                                               <  2:258857/81‑1 (MQ=255)                                 gtTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCggg                                             >  1:258923/1‑81 (MQ=255)                                 gtTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCggg                                             <  2:258858/81‑1 (MQ=255)                                        aTGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTg                                      <  2:258860/81‑1 (MQ=255)                                        aTGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTg                                      <  2:258859/81‑1 (MQ=255)                                        aTGTCGCCATATATACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTg                                      <  1:258861/81‑1 (MQ=255)                                               cATATTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAAttt                               <  2:258862/81‑1 (MQ=255)                                                atatTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTc                              <  2:258863/81‑1 (MQ=255)                                                 tatTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCa                             <  1:258864/81‑1 (MQ=255)                                                  atTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAg                            >  1:258901/1‑81 (MQ=255)                                                  atTTACGGATCGGCGAAGTCCAGGTGGCGGATGCTCCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAg                            >  2:258903/1‑81 (MQ=255)                                                      aCGGATCGGCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCaaa                        >  2:258899/1‑81 (MQ=255)                                                             ggCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTg                  >  2:258900/1‑80 (MQ=255)                                                              gCGAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTgg                 >  2:258911/1‑80 (MQ=255)                                                                gAAGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAg              >  1:258891/1‑81 (MQ=255)                                                                  aGTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGc             >  2:258904/1‑80 (MQ=255)                                                                   gTCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGcga           >  2:258914/1‑81 (MQ=255)                                                                    tCCAGGTGGCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGcgac          <  2:258865/81‑1 (MQ=255)                                                                            gCGGATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCgg  >  2:258892/1‑81 (MQ=255)                                                                               |                                                                              TCGCCTTTGATAACCGCTTTCAGCAGACGGTGGTTGATCATGTCGCCATATTTACGGATCGGCGAAGTCCAGGTGGCGTATGCTTCCAGACCGAGGCCAAAGTGAGGACCGGGTTCAGTGCTAATTTCAGCAAATGACTGGAAGCGACGAATGCGG  >  NC_000913/1347360‑1347515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑