Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,349,016	T→C	I364M (ATA→ATG)	yciW ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,349,016	0	T	C	100.0%	40.8 / NA	13	I364M (ATA→ATG)	yciW	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/3);  total (10/3)

CAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTC  >  NC_000913/1348947‑1349081                                                                      |                                                                  cAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTCATCCAGCcaca                                                        <  1:259125/81‑1 (MQ=255)    ttttACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTCATCCAGCCACACAg                                                     >  2:259141/1‑81 (MQ=255)     tttACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTCATCCAGCCACACAGc                                                    >  2:259138/1‑81 (MQ=255)           cgAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTCATCCAGCCACACAGCCCGCTc                                              >  2:259145/1‑81 (MQ=255)                  gtctgACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTCATCCAGCCACACAGCCCGCTCCATAc                                         >  2:259159/1‑79 (MQ=255)                        cgcgGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTCATCCAGCCACACAGCCCGCTCCATACCAGCAGAt                                 >  2:259146/1‑81 (MQ=255)                           ggCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTCATCCAGCCACACAGCCCGCTCCATACCAGCAGATtaa                              >  2:259140/1‑81 (MQ=255)                              aaGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTCATCCAGCCACACAGCCCGCTCCATACCAGCAGATtaataa                           >  2:259142/1‑81 (MQ=255)                                  ttAATATGTCTCACCCAACGCGATTTTTAAGCGATTCATCCAGCCACACAGCTCGCTCCATACCAGCAGATTAATAATCTg                       <  1:259126/81‑1 (MQ=255)                                           ctcACCCAACGCGATTTTTAAGCGATTCATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTtgatga              >  2:259143/1‑81 (MQ=255)                                                   acccgATTTTTAAGCGATTCATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCgt      <  2:259127/78‑1 (MQ=255)                                                   aCGCGATTTTTAAGCGATTCATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCgt      >  2:259154/1‑81 (MQ=255)                                                        aTTTTTAAGCGATTCATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTc  >  1:259157/1‑80 (MQ=255)                                                                      |                                                                  CAGTTTTACGCGAATCTGTCTGACGCGGCAAGGTTAATATGTCTCACCCAACGCGATTTTTAAGCGATTTATCCAGCCACACAGCCCGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTC  >  NC_000913/1348947‑1349081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑