Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,361,974	T→G	S22A (TCA→GCA)	puuR →	DNA‑binding transcriptional repressor PuuR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,361,974	0	T	G	100.0%	76.4 / NA	22	S22A (TCA→GCA)	puuR	DNA‑binding transcriptional repressor PuuR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/13);  total (9/13)

AAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTC  >  NC_000913/1361897‑1362045                                                                              |                                                                        aaGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGca                                                                       >  2:261172/1‑80 (MQ=255)   ggAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGCACaa                                                                    >  2:261171/1‑81 (MQ=255)          cAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAAcgtcgt                                                              <  1:261139/80‑1 (MQ=255)          cAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAAcgtcgt                                                              <  2:261141/80‑1 (MQ=255)          cAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAAcgtcgt                                                              <  1:261138/80‑1 (MQ=255)          cAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTg                                                             <  2:261140/81‑1 (MQ=255)          cAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTg                                                             >  2:261175/1‑81 (MQ=255)             atatGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTgccg                                                          >  1:261180/1‑81 (MQ=255)                     gatgaGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTGCCGCCGAActc                                                  >  1:261183/1‑81 (MQ=255)                             aCTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTGCCGCCGAACTCTCCGGGCt                                          <  1:261142/81‑1 (MQ=255)                              cTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTGCCGCCGAACTCTCCGGGCTg                                         <  1:261143/81‑1 (MQ=255)                               tGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTGCCGCCGAACTCTCCGGGCTGa                                        >  2:261182/1‑81 (MQ=255)                                  cgcCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTc                                     <  1:261144/81‑1 (MQ=255)                                              gCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCa                         <  1:261145/81‑1 (MQ=255)                                               cTTGTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAg                        <  2:261146/81‑1 (MQ=255)                                                  gTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGt                       <  2:261147/79‑1 (MQ=255)                                                  gTCGGAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTa                      <  1:261148/80‑1 (MQ=255)                                                    cGGAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGa                   >  2:261188/1‑81 (MQ=255)                                                     ggAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGa                   >  1:261177/1‑80 (MQ=255)                                                     ggAAATCCGCCAGCAGCAGGGGCTTGCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGa                   >  2:261176/1‑80 (MQ=255)                                                       aaaTCCGCCAGCAGCAGGGGCTTGCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAg                <  2:261149/81‑1 (MQ=255)                                                                     cagGGGCTTGCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTc  <  1:261150/81‑1 (MQ=255)                                                                              |                                                                        AAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGCCGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTC  >  NC_000913/1361897‑1362045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑