Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	189,243	C→T	L88L (CTG→CTA)	map ←	methionine aminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	189,243	0	C	T	100.0%	128.1 / NA	35	L88L (CTG→CTA)	map	methionine aminopeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/14);  total (21/14)

GATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTCAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATAGAGATGCAAACGGATTTCGGATAGC  >  NC_000913/189165‑189316                                                                               |                                                                          gaTAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTtag                                                                         >  2:43713/1‑81 (MQ=255)  aTAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTtag                                                                         >  2:43712/1‑80 (MQ=255)     aaCATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTtagcag                                                                      <  2:43669/80‑1 (MQ=255)     aaCATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTagcagc                                                                     >  1:43708/1‑81 (MQ=255)      aCATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCt                                                                    <  2:43670/81‑1 (MQ=255)         ttttCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAg                                                                 >  1:43732/1‑81 (MQ=255)          tttCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAg                                                                 >  1:43706/1‑80 (MQ=255)            tCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCa                                                               <  1:43671/80‑1 (MQ=255)            tCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCAt                                                              >  2:43716/1‑81 (MQ=255)             cGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCAt                                                              <  2:43672/80‑1 (MQ=255)              gAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCg                                                            <  2:43673/81‑1 (MQ=255)               aGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGt                                                           <  1:43676/81‑1 (MQ=255)               aGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGt                                                           <  2:43675/81‑1 (MQ=255)               aGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGt                                                           <  2:43674/81‑1 (MQ=255)                     cGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGa                                                     >  1:43710/1‑81 (MQ=255)                      gCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGa                                                     >  2:43718/1‑80 (MQ=255)                            gAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTATCATCGTCCGGGATACCGTg                                              <  2:43677/81‑1 (MQ=255)                            gAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGt                                               <  2:43678/80‑1 (MQ=255)                             aaaCCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTgg                                             >  1:43729/1‑81 (MQ=255)                                  aTCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCacac                                        >  2:43725/1‑81 (MQ=255)                                      ttGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACt                                    >  2:43707/1‑81 (MQ=255)                                         aTTACGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCa                                 >  1:43723/1‑81 (MQ=255)                                            aCGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCAtt                               >  1:43728/1‑80 (MQ=255)                                             cGGTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTa                              <  2:43679/80‑1 (MQ=255)                                              ggTGACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAAt                            <  2:43680/81‑1 (MQ=255)                                                 gACATCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATa                           >  1:43727/1‑79 (MQ=255)                                                    aTCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATAGAGAt                      <  1:43681/81‑1 (MQ=255)                                                    aTCAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATAGAGAt                      >  1:43711/1‑81 (MQ=255)                                                      cAATGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATAGAGATg                     >  1:43709/1‑80 (MQ=255)                                                         tGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATAGAGATGCaaa                 <  2:43682/81‑1 (MQ=255)                                                         tGTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATAGAGATGCaaa                 >  2:43714/1‑81 (MQ=255)                                                          gTTAACGATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATAGAGATGCAAAc                >  1:43736/1‑81 (MQ=255)                                                                gATATCGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATAGAGATGCAAACGGAttt          >  1:43733/1‑81 (MQ=255)                                                                     cGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATAGAGATGCAAACGGATTTCGGa      >  1:43747/1‑80 (MQ=255)                                                                     cGCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATAGAGATGCAAACGGATTTCGGa      >  1:43715/1‑80 (MQ=255)                                                                      gCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATAGAGATGCAAACGGATTTCGGa      >  1:43721/1‑79 (MQ=255)                                                                      gCCATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATAGAGATGCAAACGGATTTCGGAt     <  1:43683/80‑1 (MQ=255)                                                                        cATCTTTTAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATAGAGATGCAAACGGATTTCGGATAGc  >  2:43719/1‑81 (MQ=255)                                                                               |                                                                          GATAAACATTTTCGAGGTATCGCCGTGGAAACCATCTTTGATTACGGTGACATCAATGTTAACGATATCGCCATCTTTCAGCAGCTTAGCATCGTCCGGGATACCGTGGCACACCACTTCATTAATAGAGATGCAAACGGATTTCGGATAGC  >  NC_000913/189165‑189316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑