Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,370,687	G→A	V158I (GTT→ATT)	ycjM →	glucosylglycerate phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,370,687	0	G	A	100.0%	84.4 / NA	24	V158I (GTT→ATT)	ycjM	glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/11);  total (13/11)

CCATATGTCGGCAAAAAGTGAATGGTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCGTTGACCCGCAAACCGATCTCAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGC  >  NC_000913/1370611‑1370761                                                                             |                                                                           ccATATGTCGGCAAAAAGTGAATGGTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGa                                                                        <  2:262595/81‑1 (MQ=255)  cATATGTCGGCAAAAAGTGAATGGTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGAc                                                                       <  2:262596/81‑1 (MQ=255)      tGTCGGCAAAAAGTGAATGGTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCg                                                                    <  2:262597/80‑1 (MQ=255)       gTCGGCAAAAAGTGAATGGTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGc                                                                   >  1:262620/1‑80 (MQ=255)             aaaaaGTGAATGGTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGCAAACCGa                                                            >  2:262629/1‑81 (MQ=255)              aaaaGTGAATGGTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGCAAACCGAt                                                           >  2:262624/1‑81 (MQ=255)               aaaGTGAATGGTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGCAAACCGAtc                                                          <  2:262599/81‑1 (MQ=255)               aaaGTGAATGGTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGCAAACCGAtc                                                          <  1:262598/81‑1 (MQ=255)                aaGTGAATGGTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGCAAACCGAtct                                                         >  1:262630/1‑81 (MQ=255)                   tGAATGGTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGCAAACCGATCTCAg                                                      <  1:262600/81‑1 (MQ=255)                     aaTGGTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGCAAACCGATCTCAgcg                                                    <  2:262601/81‑1 (MQ=255)                         gTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGCAAACCGATCTCAGCGCCGt                                                >  2:262619/1‑81 (MQ=255)                          tttAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGCAAACCGATCTCAGCGCCGTc                                               <  1:671935/81‑1 (MQ=255)                                  cTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGCAAACCGATCTCAGCGCCGTCACTCGccc                                       >  1:262627/1‑81 (MQ=255)                                       tACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGCAAACCGATCTCAGCGCCGTCACTCGCCCgcgt                                   <  2:262603/80‑1 (MQ=255)                                       tACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGCAAACCGATCTCAGCGCCGTCACTCGCCCgcgt                                   <  1:262602/80‑1 (MQ=255)                                            caGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGCAAACCGATCTCAGCGCCGTCACTCGCCCGCGTGCGTTa                             >  1:262623/1‑81 (MQ=255)                                            caGCATCCAGGTTTTGAAGATTTTTTTATTGCCATTGACCCGCAAACCGATCTCAGCGCCGTCACTCGCCCGCGTGCGTTa                             <  1:262604/81‑1 (MQ=255)                                                         ttGAAGATTTTTTTATTGCCATTGACCCGCAAACCGATCTCAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGc                >  1:262622/1‑81 (MQ=255)                                                           gAAGATTTTTTTATTGCCATTGACCCGCAAACCGATCTCAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCa              >  2:262617/1‑81 (MQ=255)                                                                 ttttttATTGCCATTGACCCGCAAACCGATCTCAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTc           >  1:262659/1‑78 (MQ=255)                                                                     ttATTGCCATTGACCCGCAAACCGATCTCAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATgc    >  1:262628/1‑81 (MQ=255)                                                                       aTTGCCATTGACCCGCAAACCGATCTCAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATgcgc  >  2:262632/1‑81 (MQ=255)                                                                        ttGCCATTGACCCGCAAACCGATCTCAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATgcgc  >  1:262633/1‑80 (MQ=255)                                                                             |                                                                           CCATATGTCGGCAAAAAGTGAATGGTTTAAAAACTATTTACAACAGCATCCAGGTTTTGAAGATTTTTTTATTGCCGTTGACCCGCAAACCGATCTCAGCGCCGTCACTCGCCCGCGTGCGTTACCGTTATTAACGCCATTCCAGATGCGC  >  NC_000913/1370611‑1370761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑