Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,371,717	T→C	V501A (GTA→GCA)	ycjM →	glucosylglycerate phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,371,717	0	T	C	100.0%	54.8 / NA	16	V501A (GTA→GCA)	ycjM	glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/12);  total (4/12)

ATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATT  >  NC_000913/1371644‑1371787                                                                          |                                                                       aTTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGCAATGCg                                                                  >  2:262775/1‑80 (MQ=255)     cacTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGCAATGCGTAtt                                                              <  2:262751/80‑1 (MQ=255)           gtcgCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGCAATGCGTATTCCaag                                                         >  1:262773/1‑79 (MQ=255)                  ccATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGCAATGCGTATTCCAAGAAGTAACGc                                                <  1:262752/81‑1 (MQ=255)                    aTAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGCAATGCGTATTCCAAGAAGTAACGCTg                                              <  1:262754/81‑1 (MQ=255)                    aTAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGCAATGCGTATTCCAAGAAGTAACGCTg                                              <  2:262753/81‑1 (MQ=255)                                    ggATAATAATTTTACCATTGATACGATTAATTCATCCGCAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGAc                              >  2:262776/1‑81 (MQ=255)                                       taataaTTTTACCATTGATACGATTAATTCATCCGCAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTg                            >  1:262780/1‑80 (MQ=255)                                            atTTTACCATTGATACGATTAATTCATCCGCAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTg                       <  1:262756/80‑1 (MQ=255)                                            atTTTACCATTGATACGATTAATTCATCCGCAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTg                       <  2:262755/80‑1 (MQ=255)                                               ttACCATTGATACGATTAATTCATCCGCAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGttt                    <  2:262757/80‑1 (MQ=255)                                                tACCATTGATACGATTAATTCATCCGCAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTa                   <  2:262758/80‑1 (MQ=255)                                                        aTACGATTAATTCATCCGCAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGt           <  1:262759/80‑1 (MQ=255)                                                             aTTAATTCATCCGCAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAat     <  1:262760/81‑1 (MQ=255)                                                              ttAATTCATCCGCAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAata    <  2:262761/81‑1 (MQ=255)                                                                 aTTCATCCGCAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATAtt  <  1:262762/80‑1 (MQ=255)                                                                          |                                                                       ATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCGTATTCCAAGAAGTAACGCTGATGGTAATTGTCTGACTGGATTGTTTAATGTCAGTAAAAATATT  >  NC_000913/1371644‑1371787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑