Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,375,307	C→T	V115V (GTC→GTT)	ycjQ →	putative zinc‑binding dehydrogenase YcjQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,375,307	0	C	T	100.0%	89.5 / NA	25	V115V (GTC→GTT)	ycjQ	putative zinc‑binding dehydrogenase YcjQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/18);  total (7/18)

TATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTCATCATTAACGCAGTGAATAACTACAAGCTGCGCAAAATGCCGCAAGGCAGCTCCTGGAAAAACGCCGTCTGCT  >  NC_000913/1375229‑1375380                                                                               |                                                                          tATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGttat                                                                         >  1:263342/1‑81 (MQ=255)  aTCGAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGttat                                                                         >  2:263354/1‑80 (MQ=255)     gAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTtatcat                                                                      >  1:263346/1‑80 (MQ=255)     gAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCAtt                                                                     <  2:263307/81‑1 (MQ=255)        tGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAAc                                                                  <  2:263309/81‑1 (MQ=255)        tGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAAc                                                                  <  2:263308/81‑1 (MQ=255)           ggCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACg                                                                 >  1:263352/1‑79 (MQ=255)            gCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAg                                                              >  1:263340/1‑81 (MQ=255)               gCGACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTg                                                            >  2:263344/1‑80 (MQ=255)                 gACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAAt                                                         <  2:263310/81‑1 (MQ=255)                   cGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATaa                                                       <  1:263311/81‑1 (MQ=255)                        ccGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTAc                                                   <  2:263315/80‑1 (MQ=255)                        ccGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTAc                                                   <  2:263314/80‑1 (MQ=255)                        ccGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTACa                                                  <  1:263312/81‑1 (MQ=255)                        ccGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTACa                                                  <  1:263313/81‑1 (MQ=255)                                gcgGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTACAAGCTgcg                                           >  1:263347/1‑80 (MQ=255)                                 cgGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTACAAGCTGCGCa                                         <  1:263317/81‑1 (MQ=255)                                 cgGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTACAAGCTGCGCa                                         <  1:263316/81‑1 (MQ=255)                                  ggTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTACAAGCTGCGCaa                                        <  1:263318/81‑1 (MQ=255)                                      ggCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTACAAGCTGCGCAAAATg                                    >  1:263357/1‑81 (MQ=255)                                       gCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTACAAGCTGCGCAAAATGc                                   >  1:263348/1‑81 (MQ=255)                                        cGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTACAAGCTGCGCAAAATGcc                                  <  2:263319/81‑1 (MQ=255)                                        cGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTACAAGCTGCGCAAAATGcc                                  <  2:263320/81‑1 (MQ=255)                                                       cTACGGCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTACAAGCTGCGCAAAATGCCGCAAGGCAGCTCCTg                   >  1:263349/1‑81 (MQ=255)                                                           ggCCCGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTACAAGCTGCGCAAAATGCCGCAAGGCAGCTCCTGGaaa               <  1:263321/81‑1 (MQ=255)                                                             cccGCTCTCCGAGACGGTTATCATTAACGCAGTGAATAACTACAAGCTGCGCAAAATGCCGCAAGGCAGCTCCTGGaaaaa             <  2:263322/81‑1 (MQ=255)                                                                      cGAGACGGTTATCATTAACGCAGTGAATAACTACAAGCTGCGCAAAATGCCGCAAGGCAGCTCCTGGAAAAACGCCGTc      <  2:263323/79‑1 (MQ=255)                                                                        agaCGGTTATCATTAACGCAGTGAATAACTACAAGCTGCGCAAAATGCCGCAAGGCAGCTCCTGGAAAAACGCCGTCTGCt  <  1:263324/81‑1 (MQ=255)                                                                               |                                                                          TATCGAGTGCGGCAGCGACGTTACCGACTACGCGGTGGGCGACAGCGTATGCGGCTACGGCCCGCTCTCCGAGACGGTCATCATTAACGCAGTGAATAACTACAAGCTGCGCAAAATGCCGCAAGGCAGCTCCTGGAAAAACGCCGTCTGCT  >  NC_000913/1375229‑1375380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑