Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,399,667	T→C	intergenic (‑141/+54)	fnr ← / ← ogt	DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,399,667	0	T	C	100.0%	32.7 / NA	11	intergenic (‑141/+54)	fnr/ogt	DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/3);  total (8/3)

AACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTC  >  NC_000913/1399603‑1399741                                                                 |                                                                           aaCAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTAc                                                             <  2:266980/80‑1 (MQ=255) aaCAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACa                                                            >  2:266993/1‑81 (MQ=255)                  atatTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGc                                           >  2:266994/1‑81 (MQ=255)                    atTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTg                                         >  1:267002/1‑81 (MQ=255)                    atTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTg                                         >  1:267003/1‑81 (MQ=255)                         aTGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTCAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGcac                                     >  1:266999/1‑80 (MQ=255)                          tGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACaa                                   <  1:266981/81‑1 (MQ=255)                                          tctatTTGAAAACCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTa                    >  1:266998/1‑80 (MQ=255)                                                   aaaCCCTTAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCaa              >  2:266997/1‑77 (MQ=255)                                                          tAAGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCtt   >  2:266996/1‑81 (MQ=255)                                                            aGTTGCTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTc  <  2:266982/80‑1 (MQ=255)                                                                 |                                                                           AACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTC  >  NC_000913/1399603‑1399741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑