Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,404,016	C→T	E184K (GAA→AAA)	abgA ←	p‑aminobenzoyl‑glutamate hydrolase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,404,016	0	C	T	100.0%	99.5 / NA	28	E184K (GAA→AAA)	abgA	p‑aminobenzoyl‑glutamate hydrolase subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/12);  total (16/12)

AATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTCCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTCCGGACTCGAACTGTTTAAGGGTATGCGCCAGCC  >  NC_000913/1403939‑1404090                                                                              |                                                                           aaTGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTtctc                                                                         <  2:267616/81‑1 (MQ=255)    gtgCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTg                                                                       >  1:267653/1‑80 (MQ=255)         cGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCt                                                                 <  2:267617/81‑1 (MQ=255)               tAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGaaaaa                                                           <  2:267618/81‑1 (MQ=255)                   ataatCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAg                                                       >  2:267659/1‑81 (MQ=255)                    taatCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGt                                                      >  2:267669/1‑81 (MQ=255)                      atCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGttt                                                    >  2:267661/1‑81 (MQ=255)                       tCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTtg                                                   <  2:267619/81‑1 (MQ=255)                          aCATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTtgatg                                                <  2:267620/81‑1 (MQ=255)                            atcatcTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGAc                                              >  1:267663/1‑81 (MQ=255)                               atcTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGcc                                           <  2:267622/81‑1 (MQ=255)                               atcTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGc                                            <  1:267621/80‑1 (MQ=255)                                   aCGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATg                                        >  1:267656/1‑80 (MQ=255)                                     gACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAg                                     >  1:267660/1‑81 (MQ=255)                                     gACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAg                                     >  2:267662/1‑81 (MQ=255)                                      acacCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGt                                    >  2:267654/1‑81 (MQ=255)                                        acCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTcc                                  <  2:267623/81‑1 (MQ=255)                                            gCATCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTCCGGAc                              >  1:267664/1‑81 (MQ=255)                                              aTCGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTCCGGACTc                            <  1:267624/81‑1 (MQ=255)                                                cGACCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTCCGGACTCg                           >  2:267657/1‑80 (MQ=255)                                                  aCCATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTCCGGACTCGAAc                        <  1:267625/81‑1 (MQ=255)                                                   ccATCGCCCGCGCGCCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTCCGGACTCGAACt                       <  2:267626/81‑1 (MQ=255)                                                            gcgcgcCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTCCGGACTCGAACTGTTTAAggg              >  1:267667/1‑81 (MQ=255)                                                              gcgcCACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTCCGGACTCGAACTGTTTAAGGGTa            >  2:267671/1‑81 (MQ=255)                                                                 ccACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTCCGGACTCGAACTGTTTAAGGGTATgc         <  2:267627/81‑1 (MQ=255)                                                                  cACGCGTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTCCGGACTCGAACTGTTTAAGGGTATgcg        >  1:267673/1‑81 (MQ=255)                                                                      cgTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTCCGGACTCGAACTGTTTAAGGGTATGCGCCag    <  1:267628/81‑1 (MQ=255)                                                                       gTACCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTCCGGACTCGAACTGTTTAAGGGTATGCGCCagc   >  2:267665/1‑81 (MQ=255)                                                                         aCCTTTCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTCCGGACTCGAACTGTTTAAGGGTATGCGCCagcc  >  2:267677/1‑80 (MQ=255)                                                                              |                                                                           AATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGCGTACCTTCCTCTGCAGGCTGAAAAATCAGTTTGATGACGCCATGTAGTCCGGACTCGAACTGTTTAAGGGTATGCGCCAGCC  >  NC_000913/1403939‑1404090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑