Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,415,957	T→C	K477R (AAA→AGA)	recE ←	Rac prophage; exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,415,957	0	T	C	100.0%	117.5 / NA	33	K477R (AAA→AGA)	recE	Rac prophage; exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/19);  total (14/19)

ATATCTGGGCTGGTTTTTTCCGTTTCTGGCTGGTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATTTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGTCATCGACAGGA  >  NC_000913/1415882‑1416034                                                                            |                                                                              atatCTGGGCTGGTTTTTTCCGTTTCTGGCTGGTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGa                                                                          >  2:269266/1‑81 (MQ=255)        ggCTGGTTTTTTCCGTTTCTGGCTGGTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTc                                                                    <  1:269239/80‑1 (MQ=255)        ggCTGGTTTTTTCCGTTTCTGGCTGGTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCg                                                                   <  1:269237/81‑1 (MQ=255)        ggCTGGTTTTTTCCGTTTCTGGCTGGTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCg                                                                   <  2:269238/81‑1 (MQ=255)          cTGGTTTTTTCCGTTTCTGGCTGGTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCggg                                                                 <  2:269240/81‑1 (MQ=255)             gTTTTTTCCGTTTCTGGCTGGTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGt                                                                <  2:269241/79‑1 (MQ=255)             gTTTTTTCCGTTTCTGGCTGGTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGt                                                                <  1:269242/79‑1 (MQ=255)             gTTTTTTCCGTTTCTGGCTGGTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTc                                                               <  1:269243/80‑1 (MQ=255)                ttttCCGTTTCTGGCTGGTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCt                                                            <  2:269244/80‑1 (MQ=255)                 tttCCGTTTCTGGCTGGTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTa                                                           <  2:269245/80‑1 (MQ=255)                  ttCCGTTTCTGGCTGGTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTaa                                                          <  1:269246/80‑1 (MQ=255)                        ttCTGGCTGGTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGt                                                    >  2:269281/1‑80 (MQ=255)                             gctggTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTcaacaa                                              <  1:269247/81‑1 (MQ=255)                             gctggTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTcaaca                                               <  2:269249/80‑1 (MQ=255)                             gctggTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTcaac                                                <  2:269248/79‑1 (MQ=255)                                   tCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTcac                                        >  2:269269/1‑81 (MQ=255)                                       gTACACACAATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACg                                    >  2:269284/1‑81 (MQ=255)                                           acacaATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGAt                                <  2:269250/81‑1 (MQ=255)                                           acacaATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGAt                                >  2:269271/1‑81 (MQ=255)                                              caATCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATg                               >  2:269285/1‑79 (MQ=255)                                               aaTCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATgcag                            <  2:269251/81‑1 (MQ=255)                                                 tCGCGAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATgcagca                          <  1:269252/81‑1 (MQ=255)                                                    cgAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCagcaagc                       <  1:269253/81‑1 (MQ=255)                                                     gAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCagcaagc                       >  2:269268/1‑80 (MQ=255)                                                     gAGTCTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCagcaagc                       >  2:269290/1‑80 (MQ=255)                                                         cTGGATCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAAtt                   >  1:269279/1‑80 (MQ=255)                                                             aTCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACGAATTCACCACGTGATGCAGCAAGCAATTTATCg              <  1:269254/81‑1 (MQ=255)                                                              tCCCCTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGt             >  1:269289/1‑81 (MQ=255)                                                               ccccTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGt             >  1:269283/1‑80 (MQ=255)                                                               ccccTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGt             >  1:269273/1‑80 (MQ=255)                                                                cccTTTACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGTc            <  2:269255/80‑1 (MQ=255)                                                                     tACCCATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGTCATCGa       >  2:269297/1‑80 (MQ=255)                                                                         cATCTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGTCATCGACAGGa  >  1:269288/1‑81 (MQ=255)                                                                            |                                                                              ATATCTGGGCTGGTTTTTTCCGTTTCTGGCTGGTTCTGGTACACACAATCGCGAGTCTGGATCCCCTTTACCCATTTCGGATCGTTCGGGTCGCTAATTCCGTCAACAAATTCACCACGTGATGCAGCAAGCAATTTATCGTCATCGACAGGA  >  NC_000913/1415882‑1416034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑