Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	191,485	A→G	E210G (GAA→GGA)	tsf →	protein chain elongation factor EF‑Ts

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	191,485	0	A	G	100.0%	101.3 / NA	29	E210G (GAA→GGA)	tsf	protein chain elongation factor EF‑Ts
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/15);  total (14/15)

ACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTT  >  NC_000913/191410‑191561                                                                            |                                                                             aCGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCg                                                                         >  1:44195/1‑81 (MQ=255)  cGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGc                                                                        <  2:44154/81‑1 (MQ=255)      tCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCagag                                                                    <  1:44155/81‑1 (MQ=255)           tgcaGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGTAAAt                                                               >  2:44187/4‑81 (MQ=255)           tGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAAt                                                               >  2:44210/1‑81 (MQ=255)           tGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAAt                                                               >  1:44190/1‑81 (MQ=255)                tGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTg                                                          <  1:44156/81‑1 (MQ=255)                      aaaaaGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGcc                                                    <  1:44157/81‑1 (MQ=255)                        aaaGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCg                                                   >  1:44196/1‑80 (MQ=255)                           gAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATg                                               <  2:44158/81‑1 (MQ=255)                              tACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATgaag                                            <  2:44159/81‑1 (MQ=255)                                ccAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATgaaga                                           >  1:44183/1‑80 (MQ=255)                                 cAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGaaa                                         <  1:44160/81‑1 (MQ=255)                                     tACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCa                                     <  2:44161/81‑1 (MQ=255)                                        aGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCg                                  >  2:44185/1‑81 (MQ=255)                                          cTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGc                                <  1:44162/81‑1 (MQ=255)                                                atCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGtt                          >  1:44198/1‑81 (MQ=255)                                                     gATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTctct                     >  1:44202/1‑81 (MQ=255)                                                       tGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGa                   <  1:44163/81‑1 (MQ=255)                                                          aGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCg                >  2:44209/1‑81 (MQ=255)                                                           gTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCgg               >  2:44193/1‑81 (MQ=255)                                                            tCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGt              <  1:44164/81‑1 (MQ=255)                                                                gTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTc             <  1:44165/78‑1 (MQ=255)                                                                gTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAg           <  1:44166/80‑1 (MQ=255)                                                                   aGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgt       >  1:44199/1‑81 (MQ=255)                                                                   aGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcg        <  2:44167/80‑1 (MQ=255)                                                                    gCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgtt      >  2:44197/1‑81 (MQ=255)                                                                     ccGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgttc     <  2:44168/81‑1 (MQ=255)                                                                      cGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgttcg    >  2:44194/1‑81 (MQ=255)                                                                        aaaggaaTCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgttcgtt  <  2:44169/81‑1 (MQ=255)                                                                            |                                                                             ACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTT  >  NC_000913/191410‑191561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑