Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,428,030	C→G	A233P (GCC→CCC)	insH‑5 ←	Rac prophage; IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,428,030	0	C	G	100.0%	80.5 / NA	26	A233P (GCC→CCC)	insH‑5	Rac prophage; IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (18/8);  total (18/8)

GCGCGGATTCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGCCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTGCTCCTCTCCATGAAG  >  NC_000913/1427959‑1428099                                                                        |                                                                      gcgcGGATTCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGctcctc                                                              >  1:270749/1‑81 (MQ=255)    cgGATTCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTcgcg                                                           >  2:270743/1‑81 (MQ=255)     ggATTCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCgcgc                                                          >  1:270738/1‑81 (MQ=255)     ggATTCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCgcgc                                                          >  1:270752/1‑81 (MQ=255)     ggATTCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCgcgc                                                          <  2:270716/81‑1 (MQ=255)          cTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTgg                                                     >  1:270759/1‑81 (MQ=255)          cTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTgg                                                     >  2:270755/1‑81 (MQ=255)          cTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTgg                                                     >  1:270757/1‑81 (MQ=255)             cTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGgcgc                                                  <  2:270717/81‑1 (MQ=255)             cTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGgc                                                    <  2:270718/79‑1 (MQ=255)                   ggTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTg                                            >  1:270754/1‑81 (MQ=255)                    gTTTTTACCTTGCCGGGACGCCCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTgg                                           >  2:270767/1‑81 (MQ=14)                        ttACCTTGCCGGGACGCTCGGCGAACAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGc                                       >  2:270758/1‑81 (MQ=14)                                  gggACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGc                             >  2:270753/1‑81 (MQ=255)                                   ggACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCt                            >  2:270756/1‑81 (MQ=255)                                   ggACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCt                            >  1:270748/1‑81 (MQ=255)                                    gACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTg                           <  2:270719/81‑1 (MQ=37)                                      cGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTgag                         <  2:270721/81‑1 (MQ=18)                                      cGCTCGGCGATCAGCCAGTCAACATCCACCTCGGGCAGCTCCTCGCGATGTGGCGCTCCTTGGTAGCCGGCATCGGCTgag                         <  1:270720/81‑1 (MQ=11)                                           ggCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACaaa                    >  2:270751/1‑81 (MQ=255)                                              gATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTg                 >  2:270764/1‑81 (MQ=255)                                                  aGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTGctcc             <  1:270722/81‑1 (MQ=18)                                                   gCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTGctcct            >  2:270766/1‑81 (MQ=18)                                                           acaTCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTGCTCCTCTCCATGa    <  2:270723/81‑1 (MQ=25)                                                              tCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTGCTCCTCTCCATGAAg  >  2:270765/1‑80 (MQ=255)                                                                cACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTGCTCCTCTCCATGAAg  >  1:270760/1‑78 (MQ=255)                                                                        |                                                                      GCGCGGATTCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGCCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTGCTCCTCTCCATGAAG  >  NC_000913/1427959‑1428099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑