Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,429,596	C→G	A183G (GCA→GGA)	stfR →	Rac prophage; putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,429,596	0	C	G	100.0%	117.1 / NA	33	A183G (GCA→GGA)	stfR	Rac prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (15/18);  total (15/18)

GTCAGCCGGACAGGCCGCGTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAA  >  NC_000913/1429519‑1429673                                                                              |                                                                              gTCAGCCGGACAGGCCGCGTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATc                                                                            <  1:270957/81‑1 (MQ=255)     gCCGGACAGGCCGCGTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCaaaa                                                                        <  2:270958/81‑1 (MQ=255)      ccGGACAGGCCGCGTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCaaa                                                                         >  1:270995/1‑79 (MQ=255)       cGGACAGGCCGCGTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAg                                                                      >  2:270982/1‑81 (MQ=255)        ggACAGGCCGCGTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGt                                                                     >  2:271001/1‑81 (MQ=255)           cAGGCCGGGTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAAGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGtgct                                                                  <  2:270959/81‑1 (MQ=255)             ggCCGCGTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGtgctgc                                                                <  1:270960/81‑1 (MQ=255)              gCCGCGTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGcc                                                               <  1:270961/81‑1 (MQ=255)                 gcgTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCt                                                            >  1:270992/1‑81 (MQ=255)                   gtcgtcGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTg                                                           >  1:271008/1‑80 (MQ=255)                         ggCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTc                                                    <  1:270962/81‑1 (MQ=255)                          gCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTcc                                                   >  1:270983/1‑81 (MQ=255)                          gCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTcc                                                   <  1:270963/81‑1 (MQ=255)                               gtcagCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCaa                                               >  2:271014/1‑80 (MQ=255)                                tcagCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCaaaa                                             <  1:270964/81‑1 (MQ=255)                                   gCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAgc                                          <  2:270965/81‑1 (MQ=255)                                       cTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCgcgg                                      >  1:270993/1‑81 (MQ=255)                                        ttCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCgcggc                                     >  2:271000/1‑81 (MQ=255)                                          ccAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCgcggc                                     <  1:270966/79‑1 (MQ=255)                                          ccAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCgcgg                                      <  2:270967/78‑1 (MQ=255)                                            aGCGCAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCggcggc                                  >  2:270996/1‑80 (MQ=255)                                               gcAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCTTCAAAAAGCGCGGCGGccacc                              <  2:270968/81‑1 (MQ=255)                                               gcAGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGccacc                              <  2:270969/81‑1 (MQ=255)                                                 aGGAACGGCATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAg                            >  2:270998/1‑81 (MQ=255)                                                         cATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTg                    >  1:271012/1‑81 (MQ=255)                                                         cATCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTg                    >  2:271009/1‑81 (MQ=255)                                                          aTCAACAAAGGCCACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTgc                   <  1:270970/81‑1 (MQ=255)                                                                   ggCCACCGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAAc          <  2:270971/81‑1 (MQ=255)                                                                     ccACTGAAGGATCAAAAAGTGCTGCCGCTGCGGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGt        <  2:270972/81‑1 (MQ=255)                                                                     ccACTGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGt        <  2:270973/81‑1 (MQ=255)                                                                         tGAAGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAg     >  2:271011/1‑80 (MQ=255)                                                                           aaGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGaaa  >  1:271004/1‑81 (MQ=255)                                                                           aaGGATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGa    <  2:270974/79‑1 (MQ=255)                                                                              |                                                                              GTCAGCCGGACAGGCCGCGTCGTCGGCTCAGTCAGCGTCTTCCAGCGCAGGAACGGCATCAACAAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAA  >  NC_000913/1429519‑1429673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑