Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,486,323	A→G	K1088R (AAG→AGG)	hrpA →	ATP‑dependent RNA helicase HrpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,486,323	0	A	G	100.0%	72.6 / NA	21	K1088R (AAG→AGG)	hrpA	ATP‑dependent RNA helicase HrpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/11);  total (10/11)

AGCAGGCAATGTGGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGAC  >  NC_000913/1486248‑1486390                                                                            |                                                                    aGCAGGCAATGTGGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTAc                                                                >  2:279192/1‑81 (MQ=255)       cAATGTGGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAAc                                                           >  1:279183/1‑80 (MQ=255)          tgtgGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAg                                                       <  1:279149/81‑1 (MQ=255)             ggAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAGcc                                                     >  1:279194/1‑80 (MQ=255)             ggAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAGcc                                                     >  1:279191/1‑80 (MQ=255)              gAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAGCCa                                                    <  1:279150/80‑1 (MQ=255)               aaCGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAGCCAAg                                                  >  2:279193/1‑81 (MQ=255)                aCGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAGCCAAGc                                                 <  2:279151/81‑1 (MQ=255)                  ggTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAGCCAAGCTg                                               >  1:279182/1‑81 (MQ=255)                           cGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAGCCAAGCTGGGACTGTAc                                      <  1:279152/81‑1 (MQ=255)                            gTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAGCCAAGCTGGGACTGTACt                                     >  1:279181/1‑81 (MQ=255)                                    ctgctgAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAAccc                              <  1:279153/80‑1 (MQ=255)                                       ctgAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGt                            <  1:279154/79‑1 (MQ=255)                                       ctgAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTAt                          >  2:279189/1‑81 (MQ=255)                                         gAATATTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATgg                        >  1:279184/1‑81 (MQ=255)                                            tatTCCATCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCaa                     <  1:279155/81‑1 (MQ=255)                                                  aTCGCCAATCAAATATTTACATGAAAGGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCt               <  1:279156/81‑1 (MQ=255)                                                      ccAATCAAATATTTACATGAAAGGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAg           >  1:279203/1‑81 (MQ=255)                                                             aaTATTTACATGAAAGGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATcg    <  1:279157/81‑1 (MQ=255)                                                               tatTTACATGAAAGGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATcgac  <  1:279158/81‑1 (MQ=255)                                                               tatTTACATGAAAGGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATcgac  <  2:279159/81‑1 (MQ=255)                                                                            |                                                                    AGCAGGCAATGTGGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCGCCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAACCCGTATGGCAAAGTGCTGGAGCTGATCGAC  >  NC_000913/1486248‑1486390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑