| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 196,002 | C→T | S109F (TCC→TTC) | cdsA → | CDP‑diglyceride synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 196,002 | 0 | C | T | 96.9% | 107.5 / ‑3.9 | 32 | S109F (TCC→TTC) | cdsA | CDP‑diglyceride synthetase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (13/18); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
CGCTGGTTGAAATCTCACTTTGGGCTTCGCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTCCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTGCTAACCATTGTTCCCTTCTTCTGGGGC > NC_000913/195924‑196078 | cGCTGGTTGAAATCTCACTTTGGGCTTCGCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCg < 2:45159/81‑1 (MQ=255) gCTGGTTGAAATCTCACTTTGGGCTTCGCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCg > 2:45202/1‑80 (MQ=255) cTGGTTGAAATCTCACTTTGGGCTTCGCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTCCg > 1:45195/1‑79 (MQ=255) gTTGAAATCTCACTTTGGGCTTGGCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCgcagca < 1:45160/81‑1 (MQ=255) tGAAATCTCACTTTGGGCTTCGCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAAt > 2:45217/1‑81 (MQ=255) aaTCTCACTTTGGGCTTCGCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTg > 1:45204/1‑81 (MQ=255) aCTTTGGGCTTCGCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGc < 2:45161/77‑1 (MQ=255) aCTTTGGGCTTCGCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTaa > 1:45212/1‑81 (MQ=255) ttGGGCTTCGCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAActc > 2:45193/1‑81 (MQ=255) ggCTTCGCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTa < 2:45162/80‑1 (MQ=255) ggCTTCGCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTa < 2:45163/80‑1 (MQ=255) ggCTTCGCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTa < 1:45164/80‑1 (MQ=255) gCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTCTCGCAGCAATCTGGCGTAACTCTAAAACAtt > 2:45213/1‑81 (MQ=255) ttGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCt < 1:45165/81‑1 (MQ=255) ggATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTAttt > 2:45207/1‑81 (MQ=255) ggATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTAttt > 2:45211/1‑81 (MQ=255) gATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTAtttt < 1:45166/81‑1 (MQ=255) gATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTAttt < 2:45167/80‑1 (MQ=255) tCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGc < 1:45168/80‑1 (MQ=255) gcgcTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTg > 2:45229/1‑81 (MQ=255) gcTATTGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTGc > 2:45220/1‑80 (MQ=255) ttGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTGCTAAcc < 2:45170/81‑1 (MQ=255) ttGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTGCTAAcc < 1:45169/81‑1 (MQ=255) ttGCTGGTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTGCTAAc > 1:45216/1‑80 (MQ=255) gTGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTGCTAACCATTGtt > 2:45219/1‑81 (MQ=255) tGCTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTGCTAACCATTGTTc < 2:45171/81‑1 (MQ=255) cTGTTTTACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTGCTAACCATTGTTcc < 1:45172/80‑1 (MQ=255) ttttACCCAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTGCTAACCATTGTTCCct < 2:45173/79‑1 (MQ=255) cAGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTGCTAACCATTGTTCCCTTCTTCTggg < 2:45174/81‑1 (MQ=255) aGGTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTGCTAACCATTGTTCCCTTCTTCTgggg < 2:45175/81‑1 (MQ=255) ggTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTGCTAACCATTGTTCCCTTCTTCTgggg > 2:45209/1‑80 (MQ=255) ggTTTCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTGCTAACCATTGTTCCCTTCTTCTGGGGc < 1:45176/81‑1 (MQ=255) | CGCTGGTTGAAATCTCACTTTGGGCTTCGCTGGGTTGGTGGATTGTCGCGCTATTGCTGGTGCTGTTTTACCCAGGTTCCGCAGCAATCTGGCGTAACTCTAAAACATTGCGCCTTATTTTTGGCGTGCTAACCATTGTTCCCTTCTTCTGGGGC > NC_000913/195924‑196078 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |