Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,488,501	T→C	I90I (ATT→ATC)	aldA →	aldehyde dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,488,501	0	T	C	100.0%	55.9 / NA	17	I90I (ATT→ATC)	aldA	aldehyde dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/5);  total (12/5)

GAACGCGCCAGTTGGTTGCGCAAAATCTCCGCCGGGATCCGCGAACGCGCCAGTGAAATCAGTGCGCTGATTGTTGAAGAAGGGGGCAAGATCCAGCAGCTGGCTGAAGTCGAAGTGGCTTTTACTGCCGACTATATCGATTA  >  NC_000913/1488430‑1488572                                                                        |                                                                        gAACGCGCCAGTTGGTTGCGCAAAATCTCCGCCGGGATCCGCGAACGCGCCAGTGAAATCAGTGCGCTGATCGTTgaag                                                                  >  1:279513/1‑79 (MQ=255)         cAGTTGGTTGCGCAAAATCTCCGCCGGGATCCGCGAACGCGCCAGTGAAATCAGTGCGCTGATCGTTGAAGAAGGGGGc                                                          >  2:279503/1‑79 (MQ=255)              ggttgCGCAAAATCTCCGCCGGGATCCGCGAACGCGCCAGTGAAATCAGTGCGCTGATCGTTGAAGAAGGGGGCAAGATc                                                    >  1:279511/1‑80 (MQ=255)              ggttgCGCAAAATCTCCGCCGGGATCCGCGAACGCGCCAGTGAAATCAGTGCGCTGATCGTTGAAGAAGGGGGCAAGATc                                                    >  1:279512/1‑80 (MQ=255)                  gcgcAAAATCTCCGCCGGGATCCGCGAACGCGCCAGTGAAATCAGTGCGCTGATCGTTGAAGAAGGGGGCAAGATCcag                                                 >  1:279500/1‑79 (MQ=255)                     cAAAATCTCCGCCGGGATCCGCGAACGCGCCAGTGAAATCAGTGCGCTGATCGTTGAAGAAGGAGGCAAGATCCAGCAgct                                            <  1:279481/81‑1 (MQ=255)                       aaaTCTCCGCCGGGATCCGCGAACGCGCCAGTGAAATCAGTGCGCTGATCGTTGAAGAAGGGGGCAAGATCCAGCAgctgg                                          >  2:279499/1‑81 (MQ=255)                              cgccgGGATCCGCGAACGCGCCAGTGAAATCAGTGCGCTGATCGTTGAAGAAGGGGGCAAGATCCAGCAgctggctg                                       <  1:279482/77‑1 (MQ=255)                                 cgGGATCCGCGAACGCGCCAGTGAAATCAGTGCGCTGATCGTTGAAGAAGGGGGCAAGATCCAGCAGCTGGCTGAAGTCg                                 >  2:279496/1‑80 (MQ=255)                                        cgcgAACGCGCCAGTGAAATCAGTGCGCTGATCGTTGAAGAAGGGGGCAAGATCCAGCAGCTGGCTGAAGTCGAAGTGGCt                         <  1:279483/81‑1 (MQ=255)                                         gcgAACGCGCCAGTGAAATCAGTGCGCTGATCGTTGAAGAAGGGGGCAAGATCCAGCAGCTGGCTGAAGTCGAAGTGGCtt                        <  2:279484/81‑1 (MQ=255)                                           gAACGCGCCAGTGAAATCAGTGCGCTGATCGTTGAAGAAGGGGGCAAGATCCAGCAGCTGGCTGAAGTCGAAGTGGCttt                       >  1:279516/1‑80 (MQ=255)                                           gAACGCGCCAGTGAAATCAGTGCGCTGATCGTTGAAGAAGGGGGCAAGATCCAGCAGCTGGCTGAAGTCGAAGTGGCttt                       >  2:279508/1‑80 (MQ=255)                                            aaCGCGCCAGTGAAATCAGTGCGCTGATCGTTGAAGAAGGGGGCAAGATCCAGCAGCTGGCTGAAGTCGAAGTGGCTTTTa                     >  2:279507/1‑81 (MQ=255)                                              cgcgCCAGTGAAATCAGTGCGCTGATCGTTGAAGAAGGGGGCAAGATCCAGCAGCTGGCTGAAGTCGAAGTGGCTTTTACt                   >  2:279514/1‑81 (MQ=255)                                                       gAAATCAGTGCGCTGATCGTTGAAGAAGGGGGCAAGATCCAGCAGCTGGCTGAAGTCGAAGTGGCTTTTACTGCCGACta           >  2:279510/1‑80 (MQ=255)                                                               tGCGCTGATCGTTGAAGAAGGGGGCAAGATCCAGCAGCTGGCTGAAGTCGAAGTGGCTTTTACTGCCGACTATATCGATTa  <  1:279485/81‑1 (MQ=255)                                                                        |                                                                        GAACGCGCCAGTTGGTTGCGCAAAATCTCCGCCGGGATCCGCGAACGCGCCAGTGAAATCAGTGCGCTGATTGTTGAAGAAGGGGGCAAGATCCAGCAGCTGGCTGAAGTCGAAGTGGCTTTTACTGCCGACTATATCGATTA  >  NC_000913/1488430‑1488572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑