| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,497,760 | A→C | D302A (GAT→GCT) | opgD → | glucan biosynthesis protein D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,497,760 | 0 | A | C | 100.0% | 57.5 / NA | 17 | D302A (GAT→GCT) | opgD | glucan biosynthesis protein D |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (10/7); total (10/7) | |||||||||||
CGATGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGATCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTCAATG > NC_000913/1497682‑1497837 | cGATGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTc > 1:280720/1‑81 (MQ=255) gTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCa < 1:280705/81‑1 (MQ=255) tATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCAt > 1:280730/1‑81 (MQ=255) aTGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCAtg > 2:280735/1‑81 (MQ=255) aGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCgg > 2:280723/1‑81 (MQ=255) cTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCgggg > 1:280717/1‑81 (MQ=255) cTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCgggg > 1:280724/1‑81 (MQ=255) cGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCa < 1:280707/80‑1 (MQ=255) cGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCa < 2:280706/80‑1 (MQ=255) ggTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAAc < 2:280708/81‑1 (MQ=255) cTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCg < 2:280709/81‑1 (MQ=255) tGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTg > 1:280742/1‑81 (MQ=255) gTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGAttt < 2:280710/81‑1 (MQ=255) tgCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTcc < 2:280711/80‑1 (MQ=255) cATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCg > 2:280734/1‑81 (MQ=255) ctGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTCAATg > 1:280728/1‑81 (MQ=255) ctGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTCAATg > 2:280737/1‑81 (MQ=255) | CGATGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGATCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTCAATG > NC_000913/1497682‑1497837 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |