Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,520,784	C→T	P175S (CCA→TCA)	mcbR →	DNA‑binding transcriptional dual regulator McbR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,520,784	0	C	T	100.0%	30.9 / NA	10	P175S (CCA→TCA)	mcbR	DNA‑binding transcriptional dual regulator McbR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/3);  total (7/3)

CCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATGGGGCCTGGTTTACATTATCTCTATGAAGCGATTAATCCAGCGGAATTACGGGAGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGAT  >  NC_000913/1520707‑1520859                                                                              |                                                                            ccATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATGGGGCCTGGTTTACATTATCTCTATGAAGCGATCAATTCAg                                                                          >  2:283840/1‑81 (MQ=255)         tgtgAGATGATTGAGCAACTGTGGGTCAGGATGGGGCCTGGTTTACATTATCTCTATGAAGCGATTAATTCAGCGGAAt                                                                    <  1:283822/79‑1 (MQ=255)          gtgAGATGATTGAGCAACTGTGGGTCAGGATGGGGCCTGGTTTACATTATCTCTATGAAGCGATTAATTCAGCGGAATTAc                                                                 >  1:283845/1‑81 (MQ=255)                        cAACTGTGGGTCAGGATGGGGCCTGGTTTACATTATCTCTATGAAGCGATTAATTCAGCGGAATTACGGGAGCATATAGa                                                    >  1:283852/1‑80 (MQ=255)                         aaCTGTGGGTCAGGATGGGGCCTGGTTTACATTATCTCTATGAAGCGATTAATTCAGCGGAATTACGGGAGCATATAGaa                                                   >  1:283850/1‑80 (MQ=255)                                 gTCAGGATGGGGCCTGGTTTACATTATCTCTATGAAGCGATTAATTCAGCGGAATTACGGGAGCATATAGAAAACTatcat                                          >  1:283844/1‑81 (MQ=255)                                                      cATTATCTCTATGAAGCGATTAATTCAGCGGAATTACGGGAGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTaa                       >  1:283842/1‑79 (MQ=255)                                                       aTTATCTCTATGAAGCGATTAATTCAGCGGAATTACGGGAGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAg                    <  1:283823/81‑1 (MQ=255)                                                                      cGATTAATTCAGCGGAATTACGGGAGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAgg     >  2:283846/1‑81 (MQ=255)                                                                         ttAATTCAGCGGAATTACGGGAGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGAt  <  1:283824/81‑1 (MQ=255)                                                                              |                                                                            CCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGATGGGGCCTGGTTTACATTATCTCTATGAAGCGATTAATCCAGCGGAATTACGGGAGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGAT  >  NC_000913/1520707‑1520859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑