Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,520,982	A→G	I695T (ATT→ACT)	yncD ←	putative TonB‑dependent outer membrane receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,520,982	0	A	G	100.0%	30.8 / NA	10	I695T (ATT→ACT)	yncD	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/4);  total (6/4)

TTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATG  >  NC_000913/1520904‑1521053                                                                               |                                                                        ttATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAgta                                                                       >  2:283864/1‑81 (MQ=255)  tataCCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAgtat                                                                      >  2:283867/1‑81 (MQ=255)                    gTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGACACCATaa                                                     <  2:283843/80‑1 (MQ=255)                             aaTGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCgg                                           <  2:283844/81‑1 (MQ=255)                                  ccGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCg                                        >  1:283859/1‑79 (MQ=255)                                    ggCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAgg                                    >  1:283866/1‑81 (MQ=255)                                       aaaTACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAGTATTCATGTCGACACCATAATTTCGTCCGGGCGAAGGTTc                                 >  2:283868/1‑81 (MQ=255)                                                     aaGGTAATTACTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGt                   >  1:283858/1‑81 (MQ=255)                                                          aaTTACTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCTGGGCGAAGGTTCGTAATATCGCCCGTTTGAc              >  2:283861/1‑81 (MQ=255)                                                               cTCAAATCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCAtt         <  2:283845/81‑1 (MQ=255)                                                                    aTCTCCACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACaa    >  2:283865/1‑81 (MQ=255)                                                                         cACGCAGTATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATg  <  1:283846/78‑1 (MQ=255)                                                                               |                                                                        TTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATG  >  NC_000913/1520904‑1521053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑