Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	199,378	G→A	S484N (AGC→AAC)	bamA →	outer membrane protein assembly factor BamA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	199,378	0	G	A	100.0%	118.4 / NA	33	S484N (AGC→AAC)	bamA	outer membrane protein assembly factor BamA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/15);  total (18/15)

TCAACGGGACCAAAAACGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAGCCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACGCCGACCTGTCCGACTATACCAACAAGAGTTATG  >  NC_000913/199300‑199455                                                                               |                                                                              tCAACGGGACCAAAAACGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAAcc                                                                             <  1:45899/81‑1 (MQ=255)   aaCGGGACCAAAAACGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCt                                                                            <  1:45900/80‑1 (MQ=255)        gACCAAAAACGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCggtgg                                                                      >  1:45941/1‑81 (MQ=255)          ccAAAAACGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGgtc                                                                    <  1:45901/81‑1 (MQ=255)           cAAAAACGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGgtcg                                                                   <  2:45902/81‑1 (MQ=255)            aaaaaCGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGgtcgt                                                                  >  1:45935/1‑81 (MQ=255)            aaaaaCGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGgtcgt                                                                  >  1:45930/1‑81 (MQ=255)              aaaCGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGtct                                                                <  1:45903/81‑1 (MQ=255)                aCGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTctct                                                              >  2:45936/1‑81 (MQ=255)                     tACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCta                                                          >  2:45952/1‑80 (MQ=255)                      aCCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCtat                                                         >  2:45946/1‑80 (MQ=255)                      aCCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCtat                                                         <  2:45904/80‑1 (MQ=255)                        cAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAAt                                                      >  1:45949/1‑81 (MQ=255)                         aGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATg                                                     <  1:45906/81‑1 (MQ=255)                         aGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATg                                                     <  2:45905/81‑1 (MQ=255)                             cTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACtt                                                 >  1:45958/1‑81 (MQ=255)                                tGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTc                                                <  1:45907/79‑1 (MQ=255)                                     aaCTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGc                                           <  2:45908/79‑1 (MQ=255)                                       cTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGAt                                       >  1:45942/1‑81 (MQ=255)                                          tCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATg                                      <  2:45909/79‑1 (MQ=255)                                           cGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACg                                   >  2:45948/1‑81 (MQ=255)                                            ggTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACg                                   <  2:45910/80‑1 (MQ=255)                                             gTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACGcc                                 <  1:45911/81‑1 (MQ=255)                                             gTAACCAACCCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACGcc                                 >  1:45939/1‑81 (MQ=255)                                               aaccaaccCGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACGCCGa                               >  2:45945/1‑81 (MQ=255)                                                    accCGTACTTCACCGTAGATAGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACGCCGACCTGt                          <  1:45912/81‑1 (MQ=255)                                                     cccGTACTTCACCGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACGCCGACCTGTc                         >  1:45957/1‑81 (MQ=255)                                                                 cGTAGATGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACGCCGACCTGTCCGACTATACcaa             <  1:45913/81‑1 (MQ=255)                                                                      aTGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACGCCGACCTGTCCGACTATACcaacaa          >  2:45944/1‑79 (MQ=255)                                                                      aTGGCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACGCCGACCTGTCCGACTATACcaacaa          >  2:45947/1‑79 (MQ=255)                                                                        ggCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACGCCGACCTGTCCGACTATACCAACAAGAGt      >  1:45954/1‑81 (MQ=255)                                                                         gCGTAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACGCCGACCTGTCCGACTATACCAACAAGAGt      >  2:45943/1‑80 (MQ=255)                                                                            tAAACCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACGCCGACCTGTCCGACTATACCAACAAGAGTTATg  >  2:45951/1‑81 (MQ=255)                                                                               |                                                                              TCAACGGGACCAAAAACGATTACCAGACCTATGCTGAACTGTCGGTAACCAACCCGTACTTCACCGTAGATGGCGTAAGCCTCGGTGGTCGTCTCTTCTATAATGACTTCCAGGCAGATGACGCCGACCTGTCCGACTATACCAACAAGAGTTATG  >  NC_000913/199300‑199455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑