Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,534,244	G→A	G74D (GGC→GAC)	nhoA →	arylamine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,534,244	0	G	A	100.0%	99.8 / NA	29	G74D (GGC→GAC)	nhoA	arylamine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/16);  total (13/16)

AATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCG  >  NC_000913/1534167‑1534320                                                                              |                                                                             aaTACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGACgt                                                                           >  2:285607/1‑81 (MQ=255)        cTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAg                                                                    >  2:285599/1‑81 (MQ=255)          tGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCg                                                                  >  2:285608/1‑81 (MQ=255)            aTAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCgg                                                                 <  2:285571/80‑1 (MQ=255)            aTAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCgg                                                                 <  2:285570/80‑1 (MQ=255)             tAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCGGgt                                                               >  1:285604/1‑81 (MQ=255)                tcaatcGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCGGGTGtt                                                            <  2:285572/81‑1 (MQ=255)                  aatcGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCGGGTGTTAc                                                          <  1:285573/81‑1 (MQ=255)                   atcGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCGGGTGTTAcg                                                         >  2:285613/1‑81 (MQ=255)                      gCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCGGGTGTTAcgcg                                                       <  2:285574/80‑1 (MQ=255)                        cGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCGGGTGTTACGCGAGc                                                    >  2:285606/1‑81 (MQ=255)                         ggAAGAGAAACTGGTGCTAGCTCGTCGTGGCGGTTACTGTTTTGAGCAGAATGATGCGTTTGAGCGGGTTTTACGCGAGCt                                                   >  2:285615/1‑81 (MQ=255)                               gaAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGtt                                             <  2:285575/81‑1 (MQ=255)                                   cTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAAc                                         <  2:285576/81‑1 (MQ=255)                                       tGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTc                                     <  2:285577/81‑1 (MQ=255)                                                gtcgtGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGt                            >  1:285605/1‑81 (MQ=255)                                                   gtGGCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTa                          <  1:285578/80‑1 (MQ=255)                                                      gCGGTTACTGTTTTGAGCAGAATGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGgc                      <  1:285579/81‑1 (MQ=255)                                                        ggTTACTGTTTTGAGCAGAATGACGTGTTTGAGCGGGTGTTACGCGAGCCGGGGTTTAACGTTCGCAGCTTGTTAGGgcgc                    >  2:285610/1‑81 (MQ=255)                                                               gTTTTGAGCAGAATGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAgtgt             >  1:285616/1‑81 (MQ=255)                                                                 tttGAGCAGAATGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTa           >  1:285601/1‑81 (MQ=255)                                                                 tttGAGCAGAATGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTa           >  1:285603/1‑81 (MQ=255)                                                                    gAGCAGAATGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATc         <  2:285580/80‑1 (MQ=255)                                                                     aGCAGAATGACGTGTTTGAGCAGGTGTTACGCGAGCTGGGGTTTAACGTTCGCACCTTGTTAGGGCGCGTAGTGTTATCa        <  1:285581/80‑1 (MQ=255)                                                                       cAGAATGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAAt     <  2:285582/81‑1 (MQ=255)                                                                       cAGAATGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAAt     <  1:285583/81‑1 (MQ=255)                                                                        aGAATGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATc    <  1:285584/81‑1 (MQ=255)                                                                         gAATGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATcc   >  2:285602/1‑81 (MQ=255)                                                                          aaTGACGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCg  <  1:285585/81‑1 (MQ=255)                                                                              |                                                                             AATACAGCTTGATAATCAATCGCCGGAAGAGAAACTGGTGATAGCCCGTCGTGGCGGTTACTGTTTTGAGCAGAATGGCGTGTTTGAGCGGGTGTTACGCGAGCTGGGGTTTAACGTTCGCAGCTTGTTAGGGCGCGTAGTGTTATCAAATCCG  >  NC_000913/1534167‑1534320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑