Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,575,336	T→G	S762R (AGC→CGC)	yddB ←	putative TonB‑dependent receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,575,336	0	T	G	100.0%	87.1 / NA	25	S762R (AGC→CGC)	yddB	putative TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/17);  total (8/17)

TGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGG  >  NC_000913/1575261‑1575412                                                                            |                                                                             tGACATCAAGCCAGAAAGTACGGCCACTGGCGCAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATc                                                                           >  1:291213/1‑79 (MQ=255)   aCATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAg                                                                        >  1:291211/1‑80 (MQ=255)    cATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTa                                                                      >  2:291206/1‑81 (MQ=255)           ccAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACAtt                                                                  >  1:291215/1‑78 (MQ=255)             aGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGaa                                                             >  2:291224/1‑81 (MQ=255)               aaaGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAata                                                           <  2:291177/81‑1 (MQ=255)                aaGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAatat                                                          <  2:291178/81‑1 (MQ=255)                        ccACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTg                                                  <  1:291179/81‑1 (MQ=255)                           cTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATc                                               <  1:291180/81‑1 (MQ=255)                                    gTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGttt                                      <  2:291181/81‑1 (MQ=255)                                       gCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTttg                                    <  1:291182/80‑1 (MQ=255)                                        ccACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTttgtt                                  >  1:291218/1‑81 (MQ=255)                                         cacaCCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTttgttg                                 <  1:291183/81‑1 (MQ=255)                                          acacCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTttgttgt                                <  1:291184/81‑1 (MQ=255)                                           cacCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTttgttgtt                               <  2:291185/81‑1 (MQ=255)                                              cGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCa                            <  2:291186/81‑1 (MQ=255)                                                  gtTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGaaa                        >  1:291216/1‑81 (MQ=255)                                                     cGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAAtt                      <  2:291187/80‑1 (MQ=255)                                                      gTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATtgt                    <  2:291188/81‑1 (MQ=255)                                                         gTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGc                 >  2:291223/1‑81 (MQ=255)                                                          tATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGc                 <  2:291189/80‑1 (MQ=255)                                                                cAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAgg          <  2:291190/81‑1 (MQ=255)                                                                     gTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAg     <  1:291191/81‑1 (MQ=255)                                                                        ttGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCg   <  2:291192/80‑1 (MQ=255)                                                                         tGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCgg  <  2:291193/80‑1 (MQ=255)                                                                            |                                                                             TGACATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGG  >  NC_000913/1575261‑1575412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑