Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,578,871	A→G	F158L (TTC→CTC)	yddA ←	ABC transporter family protein YddA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,578,871	0	A	G	100.0%	69.3 / NA	20	F158L (TTC→CTC)	yddA	ABC transporter family protein YddA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/9);  total (11/9)

TGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTAT  >  NC_000913/1578799‑1578942                                                                         |                                                                        tGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACCGGATGAGTCCAAAt                                                                  >  1:291598/1‑80 (MQ=255)        gagCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACt                                                          <  1:311122/81‑1 (MQ=255)            gTACCCGCGCTTTCCCAAAGAATGACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGc                                                      >  2:291604/1‑81 (MQ=255)            gTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGc                                                      >  1:291595/1‑81 (MQ=255)                       ttCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGAt                                           >  2:291603/1‑81 (MQ=255)                         cccAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATc                                          <  2:291574/80‑1 (MQ=255)                            aaaGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCC‑AATGAAAGACTGAGCGTTTTGCTGATCAGt                                       >  2:291602/1‑79 (MQ=255)                                aaTAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTaa                                     >  1:291608/1‑78 (MQ=255)                                aaTAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTaa                                     >  1:291606/1‑78 (MQ=255)                                  tAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGaa                                <  2:291575/81‑1 (MQ=255)                                   aaCGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAAt                               >  1:291605/1‑81 (MQ=255)                                             gTGATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGc                      >  1:291611/1‑80 (MQ=255)                                               gATCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGa                    >  1:291601/1‑80 (MQ=255)                                                aTCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGAt                   <  1:291576/80‑1 (MQ=255)                                                aTCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGAt                   <  1:291577/80‑1 (MQ=255)                                                 tCAGCATGCTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATAc                 <  2:291578/81‑1 (MQ=255)                                                         cTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCa         <  2:291580/81‑1 (MQ=255)                                                         cTAAGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCa         <  1:291579/81‑1 (MQ=255)                                                            aGTGACTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAgg       <  1:291581/80‑1 (MQ=255)                                                                aCTGGATGAGGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTAt  >  1:291607/1‑81 (MQ=255)                                                                         |                                                                        TGAATGAGAGCGTACCCGCGCTTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTAT  >  NC_000913/1578799‑1578942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑