Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,607,828	C→T	G161G (GGC→GGT)	tam →	trans‑aconitate 2‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,607,828	0	C	T	100.0%	78.7 / NA	23	G161G (GGC→GGT)	tam	trans‑aconitate 2‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/7);  total (16/7)

GGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCAC  >  NC_000913/1607750‑1607900                                                                               |                                                                         ggAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGgtgt                                                                        >  2:295152/1‑81 (MQ=255)  gAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGgtgt                                                                        <  2:295130/80‑1 (MQ=255)       gACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGc                                                                  >  1:295147/1‑81 (MQ=255)          ccATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTta                                                               >  2:295158/1‑81 (MQ=255)                   cATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGatat                                                      >  2:295160/1‑81 (MQ=255)                   cATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGatat                                                      >  1:295161/1‑81 (MQ=255)                           aaGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGc                                               >  1:295153/1‑80 (MQ=255)                                gCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGcc                                         >  2:295163/1‑81 (MQ=255)                                       aaCAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGAtgtg                                  <  1:295131/81‑1 (MQ=255)                                         cAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAg                                >  2:295191/1‑81 (MQ=255)                                                 cccGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGatat                        <  2:295132/81‑1 (MQ=255)                                                 cccGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGat                          <  1:295133/79‑1 (MQ=255)                                                  ccGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATc                       >  2:295164/1‑81 (MQ=255)                                                   cGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCt                      <  1:295134/81‑1 (MQ=255)                                                    ggATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTg                     >  2:295167/1‑81 (MQ=255)                                                      aTCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGc                   >  1:295168/1‑81 (MQ=255)                                                      aTCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGc                   >  1:295154/1‑81 (MQ=255)                                                          cgGGCGTGAGCCGTT‑GCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAac               >  1:295174/1‑80 (MQ=255)                                                           gggCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAac               >  2:295155/1‑80 (MQ=255)                                                           gggCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAac               >  2:295162/1‑80 (MQ=255)                                                                tGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACcta         >  2:295169/1‑81 (MQ=255)                                                                    ccGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTAt     <  1:295135/81‑1 (MQ=255)                                                                     cGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATc    <  2:295136/81‑1 (MQ=255)                                                                      gTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCa   >  2:295165/1‑81 (MQ=255)                                                                       ttGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCAc  <  1:295137/81‑1 (MQ=255)                                                                               |                                                                         GGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCAC  >  NC_000913/1607750‑1607900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑