Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	205,791	C→T	G222G (GGC→GGT)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	205,791	0	C	T	100.0%	137.0 / NA	38	G222G (GGC→GGT)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/20);  total (18/20)

CGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAAGAGGAGATGTG  >  NC_000913/205713‑205865                                                                               |                                                                           cgtGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGttt                                                                          >  2:47420/1‑81 (MQ=255)  gtGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGttt                                                                          <  2:47364/80‑1 (MQ=255)  gtGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGttt                                                                          <  2:47363/80‑1 (MQ=255)      cGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTAccc                                                                     <  1:47365/81‑1 (MQ=255)      cGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTAccc                                                                     >  2:47428/1‑81 (MQ=255)       gACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCt                                                                    >  2:47408/1‑81 (MQ=255)           aaCGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTcgac                                                                >  2:47434/1‑81 (MQ=255)              gacgTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGAt                                                             >  2:47424/1‑81 (MQ=255)                cgTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATcc                                                           <  2:47367/81‑1 (MQ=255)                cgTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATcc                                                           <  2:47366/81‑1 (MQ=255)                 gTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATcc                                                           >  2:47416/1‑80 (MQ=255)                 gTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCt                                                          <  2:47368/81‑1 (MQ=255)                    cgcTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTaa                                                        <  2:47369/80‑1 (MQ=255)                     gcTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAAc                                                      <  2:47370/81‑1 (MQ=255)                        ttATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGcc                                                   >  2:47417/1‑81 (MQ=255)                        ttATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGcc                                                   >  2:47418/1‑81 (MQ=255)                         tATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGccc                                                  >  2:47427/1‑81 (MQ=255)                            cGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCcgcg                                               >  1:47412/1‑81 (MQ=255)                             gACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGt                                              <  1:47371/81‑1 (MQ=255)                                agcagcGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAAc                                           <  1:47372/81‑1 (MQ=255)                                 gcagcGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAAc                                           >  1:47433/1‑80 (MQ=255)                                     cGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTc                                      <  2:47373/81‑1 (MQ=255)                                      gACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTcg                                     >  1:47425/1‑81 (MQ=255)                                       aCTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTcgc                                    <  2:47374/81‑1 (MQ=255)                                           tGACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTcgccgc                                 <  1:47375/80‑1 (MQ=255)                                            gACGCACACGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCgca                                <  2:47376/80‑1 (MQ=255)                                                  cacGAAATCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAatat                         <  1:47377/81‑1 (MQ=255)                                                    cGAAATCCGCGTCGCGATCGACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCACAGCAatatat                       <  1:47378/81‑1 (MQ=255)                                                       aaTCCGCGTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCg                    >  2:47431/1‑81 (MQ=255)                                                           cgcgTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAg                 <  2:47379/80‑1 (MQ=255)                                                           cgcgTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAg                 <  1:47380/80‑1 (MQ=255)                                                            gcgTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGc                >  2:47423/1‑80 (MQ=255)                                                            gcgTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCg               <  1:47382/81‑1 (MQ=255)                                                            gcgTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCg               <  2:47381/81‑1 (MQ=255)                                                             cgTCGCGATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCg               >  2:47430/1‑80 (MQ=255)                                                                 gcgATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAagag          <  2:47383/81‑1 (MQ=255)                                                                  cgATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAAgagg         <  2:47384/81‑1 (MQ=255)                                                                   gATCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAagag          >  1:47435/1‑79 (MQ=255)                                                                     tCCACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAagag          >  1:47409/1‑77 (MQ=255)                                                                       cACGACGGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAAGAGGAGAtg    >  2:47422/1‑81 (MQ=255)                                                                         cgacgGTTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAAGAGGAGAtgtg  >  2:47436/1‑81 (MQ=255)                                                                               |                                                                           CGTGGCGACCAACGACGTGCGCTTTATCGACAGCAGCGACTTTGACGCACACGAAATCCGCGTCGCGATCCACGACGGCTTTACCCTCGACGATCCTAAACGCCCGCGTAACTATTCGCCGCAGCAATATATGCGTAGCGAAGAGGAGATGTG  >  NC_000913/205713‑205865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑