Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,658,706	G→C	G213A (GGC→GCC)	ynfE →	putative selenate reductase YnfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,658,706	0	G	C	100.0%	76.8 / NA	22	G213A (GGC→GCC)	ynfE	putative selenate reductase YnfE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (9/13);  total (9/13)

CTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGGCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGCG  >  NC_000913/1658633‑1658783                                                                          |                                                                              cTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGc                                                                        <  1:301152/81‑1 (MQ=255)   cAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGcac                                                                      >  2:301187/1‑81 (MQ=255)         gTATGGCTCCTAAAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGa                                                                <  1:301153/81‑1 (MQ=255)            tGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGata                                                              <  2:301154/80‑1 (MQ=255)                   tACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGaaaa                                                       >  1:301190/1‑80 (MQ=255)                   tACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGaaaa                                                       >  1:301192/1‑80 (MQ=255)                     cAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAg                                                    <  2:301155/81‑1 (MQ=255)                            gcgcAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTg                                             >  1:301193/1‑81 (MQ=255)                             cgcAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTg                                             <  2:301156/80‑1 (MQ=255)                               cAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTc                                          <  2:301157/81‑1 (MQ=255)                                       cTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGtt                                  >  2:301191/1‑81 (MQ=255)                                          tgCCATGCCCTACACCTACGACAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGATCGTGATGttt                                 <  1:301158/79‑1 (MQ=255)                                            ccATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGt                              <  2:301159/80‑1 (MQ=255)                                              aTGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTaac                           <  2:301160/81‑1 (MQ=255)                                                  ccTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAAcc                       <  2:301161/81‑1 (MQ=255)                                                     acacCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCgg                    >  1:301199/1‑81 (MQ=255)                                                            cGGCAGTAATGATGCCAACAGCACCACGGGTATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGaa               <  2:301163/79‑1 (MQ=255)                                                            cGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAAc             >  1:301195/1‑81 (MQ=255)                                                            cGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAAc             <  2:301162/81‑1 (MQ=255)                                                                  tAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCAt       >  1:301194/1‑81 (MQ=255)                                                                  tAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCAt       <  2:301164/81‑1 (MQ=255)                                                                       atgCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGCg  >  2:301198/1‑81 (MQ=255)                                                                          |                                                                              CTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGGCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGCG  >  NC_000913/1658633‑1658783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑