Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,665,852	T→C	L180L (TTG→CTG)	clcB →	putative chloride:H(+) antiporter ClcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,665,852	0	T	C	100.0%	126.4 / NA	35	L180L (TTG→CTG)	clcB	putative chloride:H(+) antiporter ClcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/23);  total (12/23)

GCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGTTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTGGTTAGCAATCTGATTAATCATAGCGA  >  NC_000913/1665774‑1665925                                                                               |                                                                          gcggGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTg                                                                         <  1:302175/81‑1 (MQ=255)     gAATGGCTGCGGCCTATCGTGCCCCGCCTGCTGGCAGTTTATTTATAGCCGACGTGCTGTTTGGCACTATGATGCTGGCct                                                                     >  1:302216/1‑81 (MQ=255)       aTGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCctct                                                                   <  2:302176/81‑1 (MQ=255)        tGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCctctc                                                                  <  1:302177/81‑1 (MQ=255)        tGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCctctc                                                                  <  2:302178/81‑1 (MQ=255)         ggCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCctctct                                                                 >  1:302220/1‑81 (MQ=255)          gCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCctctct                                                                 <  1:302179/80‑1 (MQ=255)          gCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCctctct                                                                 <  1:302180/80‑1 (MQ=255)          gCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCtctctc                                                                >  2:302208/1‑81 (MQ=255)          gCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCtctctc                                                                <  1:302181/81‑1 (MQ=255)           cTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCg                                                               <  1:302183/81‑1 (MQ=255)           cTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCg                                                               <  1:302182/81‑1 (MQ=255)               ggCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGccc                                                           <  1:302184/81‑1 (MQ=255)                     tCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTg                                                       <  2:302185/79‑1 (MQ=255)                       gTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGatta                                                   >  1:302223/1‑81 (MQ=255)                        tGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGattat                                                  >  1:302230/1‑81 (MQ=255)                           cccGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTc                                               >  1:302222/1‑81 (MQ=255)                             cGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTccg                                             <  2:302186/81‑1 (MQ=255)                                  gCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTccgccg                                          <  1:302187/79‑1 (MQ=255)                                   cTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCcgtcg                                       <  1:302188/81‑1 (MQ=255)                                      gCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTgg                                    >  1:302241/1‑81 (MQ=255)                                        aGTTTTTTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCa                                  >  2:302219/1‑81 (MQ=255)                                         gTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCAt                                 <  1:302189/81‑1 (MQ=255)                                          tttatttaTAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCAtt                                <  1:302190/81‑1 (MQ=255)                                             atttatAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGc                              <  2:302191/80‑1 (MQ=255)                                               ttatAGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTgg                           >  2:302225/1‑81 (MQ=255)                                                  taGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTGGtt                         <  1:302193/80‑1 (MQ=255)                                                  taGCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTGGtt                         <  2:302192/80‑1 (MQ=255)                                                    gCCGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTGGTTAg                       >  2:302218/1‑80 (MQ=255)                                                      cGAAGTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTGGTTAGCaa                    >  1:302221/1‑81 (MQ=255)                                                          gTGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTGGTTAGCAATCTg                <  2:302194/81‑1 (MQ=255)                                                           tGCTGTTTGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTGGTTAGCAATCTGa               <  1:302195/81‑1 (MQ=255)                                                                tttGGCACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTGGTTAGCAATCTGATTAAt          >  2:302224/1‑81 (MQ=255)                                                                     cACTATGATGCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTGGTTAGCAATCTGATTAATCATAg     <  1:302196/81‑1 (MQ=255)                                                                          tgatgCTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTGGTTAGCAATCTGATTAATCATAGCGa  <  1:302197/79‑1 (MQ=255)                                                                               |                                                                          GCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGTTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTGGTTAGCAATCTGATTAATCATAGCGA  >  NC_000913/1665774‑1665925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑