Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	208,192	T→A	S1023T (TCC→ACC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	208,192	0	T	A	100.0%	122.4 / NA	34	S1023T (TCC→ACC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/20);  total (14/20)

GCGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTT  >  NC_000913/208114‑208269                                                                               |                                                                              gCGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTAcc                                                                             >  1:48007/1‑81 (MQ=255)  cGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTAcc                                                                             <  1:47959/80‑1 (MQ=255)  cGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTAcc                                                                             <  1:47958/80‑1 (MQ=255)   ggggCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCgg                                                                           >  2:48006/1‑81 (MQ=255)     ggCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGggc                                                                         <  2:47960/81‑1 (MQ=255)         cGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCt                                                                     >  1:48015/1‑81 (MQ=255)         cGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCt                                                                     >  1:47999/1‑81 (MQ=255)          gttgttGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCt                                                                     <  1:47961/80‑1 (MQ=255)           ttgttgCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTg                                                                    >  2:48003/1‑80 (MQ=255)                gCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGt                                                               >  2:48014/1‑80 (MQ=255)                     gcgGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGAtgtt                                                         <  1:47962/81‑1 (MQ=255)                           tATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTAc                                                   >  1:48017/1‑81 (MQ=255)                               gTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGa                                                <  1:47963/80‑1 (MQ=255)                                 cACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGc                                             >  2:48048/1‑81 (MQ=255)                                     aaGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTg                                         <  1:47964/81‑1 (MQ=255)                                         gcgGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGAt                                      >  1:48016/1‑80 (MQ=255)                                         gcgGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGAt                                      >  1:48021/1‑80 (MQ=255)                                         gcgGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATa                                     <  1:47965/81‑1 (MQ=255)                                            gCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAAt                                  <  1:47966/81‑1 (MQ=255)                                              aaTCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATAc                                >  1:48010/1‑81 (MQ=255)                                                  gTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAg                             <  1:47967/80‑1 (MQ=255)                                                     tCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAAt                         <  2:47968/81‑1 (MQ=255)                                                        gTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGc                      <  2:47969/81‑1 (MQ=255)                                                               cACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTgg                   <  1:47971/77‑1 (MQ=255)                                                               cACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGaaaa               <  1:47970/81‑1 (MQ=255)                                                               cACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGaa                 <  1:47972/79‑1 (MQ=255)                                                                     ggATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCg         >  1:48030/1‑81 (MQ=255)                                                                      gATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCg         <  1:47974/80‑1 (MQ=255)                                                                      gATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGc        <  1:47973/81‑1 (MQ=255)                                                                        tGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCAt      <  2:47975/81‑1 (MQ=255)                                                                         gACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATa     >  1:48020/1‑81 (MQ=255)                                                                          aCCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATa     <  1:47976/80‑1 (MQ=255)                                                                            cGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACtt  <  2:47977/81‑1 (MQ=255)                                                                            cGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACt   >  1:48029/1‑80 (MQ=255)                                                                               |                                                                              GCGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTT  >  NC_000913/208114‑208269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑