Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	239,682	A→C	S170A (TCT→GCT)	yafV ←	2‑oxoglutaramate amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	239,682	0	A	C	100.0%	119.6 / NA	34	S170A (TCT→GCT)	yafV	2‑oxoglutaramate amidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/15);  total (19/15)

GCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTA  >  NC_000913/239605‑239759                                                                              |                                                                              gCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGcgcg                                                                            >  1:54572/1‑81 (MQ=255)  ccGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAgcgc                                                                             <  2:54525/79‑1 (MQ=255)    gACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGcgcgcg                                                                          >  1:54579/1‑80 (MQ=255)    gACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCgg                                                                         <  2:54526/81‑1 (MQ=255)     aCGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGcgcgcg                                                                          >  2:54565/1‑79 (MQ=255)          aTTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAgg                                                                   >  2:54561/1‑81 (MQ=255)             gCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGcc                                                                 <  2:54527/80‑1 (MQ=255)             gCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGcc                                                                 <  2:54528/80‑1 (MQ=255)                  ccGCCACGTACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTg                                                            <  2:54529/80‑1 (MQ=255)                  ccGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTgg                                                           >  1:54590/1‑81 (MQ=255)                    gCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGc                                                          >  1:54577/1‑80 (MQ=255)                        caTACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGt                                                     <  2:54530/81‑1 (MQ=255)                         aTACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTa                                                    >  1:54595/1‑81 (MQ=255)                          tACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTa                                                    >  2:54574/1‑80 (MQ=255)                          tACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTAc                                                   <  2:54531/81‑1 (MQ=255)                           aCGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTa                                                    >  2:54584/1‑79 (MQ=255)                            cGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAg                                                 <  1:54532/81‑1 (MQ=255)                             gCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAgg                                                >  1:54597/1‑81 (MQ=255)                              ccTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAggg                                               <  2:54533/81‑1 (MQ=255)                              ccTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAggg                                               >  1:54578/1‑81 (MQ=255)                                    tctcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGt                                         >  1:54592/1‑81 (MQ=255)                                    tctcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGt                                         >  2:54571/1‑81 (MQ=255)                                      tcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCa                                       >  2:54576/1‑81 (MQ=255)                                       cAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCa                                       >  1:54580/1‑80 (MQ=255)                                       cAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCa                                       >  2:54586/1‑80 (MQ=255)                                        aaTCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCAt                                      <  1:54535/80‑1 (MQ=255)                                        aaTCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCAt                                      <  2:54536/80‑1 (MQ=255)                                        aaTCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCAt                                      >  1:54587/1‑80 (MQ=255)                                        aaTCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATa                                     <  2:54534/81‑1 (MQ=255)                                           cgcgcgGGCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATc                                  >  2:54593/1‑81 (MQ=255)                                                gggCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGtt                               >  2:54585/1‑79 (MQ=255)                                                gggCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGtt                               >  2:54589/1‑79 (MQ=255)                                                 ggCCGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGtt                               >  2:54602/1‑78 (MQ=255)                                                    cGTCAGCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGAtt                         <  2:54537/81‑1 (MQ=255)                                                         gCAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTgcgc                     >  1:54591/1‑80 (MQ=255)                                                          cAATGCCTGCCAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGa                   <  1:54538/81‑1 (MQ=255)                                                                ctgccAGTGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACcacac             <  2:54539/81‑1 (MQ=255)                                                                       tGCAGAGCGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGaaaa      <  2:54540/81‑1 (MQ=255)                                                                           gagcgCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTa  <  2:54541/81‑1 (MQ=255)                                                                              |                                                                              GCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTA  >  NC_000913/239605‑239759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑