Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,750,974	T→A	T14S (ACA→TCA)	ydhX ←	putative 4Fe‑4S ferredoxin‑type protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,750,974	0	T	A	100.0%	64.1 / NA	19	T14S (ACA→TCA)	ydhX	putative 4Fe‑4S ferredoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/7);  total (12/7)

ATGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGT  >  NC_000913/1750898‑1751046                                                                             |                                                                         aTGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATcc                                                                       <  1:313785/80‑1 (MQ=255)     cATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATcc                                                                  >  1:313798/1‑81 (MQ=255)            aaCTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAAc                                                           <  2:313786/81‑1 (MQ=255)                   ttttCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTg                                                    >  2:313813/1‑81 (MQ=255)                       cTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGt                                                 <  1:313787/80‑1 (MQ=255)                                tgtTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAgg                                       <  2:313788/81‑1 (MQ=255)                                  ttCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGa                                      >  2:313827/1‑80 (MQ=255)                                          cAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGAt                              >  2:313808/1‑80 (MQ=255)                                           agagAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTa                            >  2:313815/1‑81 (MQ=255)                                           agagAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTa                            >  2:313816/1‑81 (MQ=255)                                                 gaCGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATcccc                       >  2:313814/1‑80 (MQ=255)                                                     cAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATg                  <  2:313789/81‑1 (MQ=255)                                                       gAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATg                  >  1:313820/1‑79 (MQ=255)                                                       gAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATg                  >  2:313819/1‑79 (MQ=255)                                                            aGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGc           >  1:313822/1‑81 (MQ=255)                                                            aGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGc           >  2:313818/1‑81 (MQ=255)                                                             gTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCt          <  2:313790/81‑1 (MQ=255)                                                                aaaaaGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCa       <  2:313791/81‑1 (MQ=255)                                                                     gATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGt  >  1:313812/1‑81 (MQ=255)                                                                             |                                                                         ATGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGT  >  NC_000913/1750898‑1751046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑