Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,754,826	C→T	intergenic (‑349/+106)	ydhY ← / ← fumD	putative 4Fe‑4S ferredoxin‑type protein/fumarase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,754,826	0	C	T	100.0%	39.4 / NA	12	intergenic (‑349/+106)	ydhY/fumD	putative 4Fe‑4S ferredoxin‑type protein/fumarase D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/4);  total (8/4)

ACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATGATTAACTGGACCGCAACT  >  NC_000913/1754755‑1754886                                                                        |                                                             aCTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATATATACCGaaa                                                     >  1:314299/1‑81 (MQ=255)    tAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATATATACCGAAATTc                                                  >  2:314324/1‑81 (MQ=255)     aaGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATATATACCGAAATTCg                                                 >  1:314304/1‑81 (MQ=255)             attattTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATATATACCGAAATTCGTATAAttt                                         >  1:314300/1‑81 (MQ=255)              ttattTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATATATACCGAAATTCGTATAATTTc                                        <  1:314287/81‑1 (MQ=255)                attTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATATATACCGAAATTCGTATAATTTcag                                      >  2:314302/1‑81 (MQ=255)                        cTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATATATACCGAAATTCGTATAATTTCAGCAgttgtt                              <  1:314288/81‑1 (MQ=255)                           tCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATATATACCGAAATTCGTATAATTTCAGCAGTTGTTAtt                           >  2:314305/1‑81 (MQ=255)                            cATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATATATACCGAAATTCGTATAATTTCAGCAGTTGTTAtt                           <  1:314289/80‑1 (MQ=255)                                  aGAAAATAAAAATAACATTATCGCTATAAATTAAAATATATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATg                    <  2:314290/81‑1 (MQ=255)                                                  aTTATCGCTATAAATTAAAATATATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATGATTAACTGGACCGCaa    >  2:314307/1‑81 (MQ=255)                                                    tATCGCTATAAATTAAAATATATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATGATTAACTGGACCGCAACt  >  2:314310/1‑81 (MQ=255)                                                                        |                                                             ACTTAAGCGACAATTATTTGTGACTTTCATTGCAGAAAATAAAAATAACATTATCGCTATAAATTAAAATACATACCGAAATTCGTATAATTTCAGCAGTTGTTATTTAGAATGATTAACTGGACCGCAACT  >  NC_000913/1754755‑1754886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑